School of Water and Environment, Chang'an University, Xi'an 710064, Shaanxi, China; Key Laboratory of Subsurface Hydrology and Ecological Effect in Arid Region (Chang'an University), Ministry of Education, Xi'an 710064, Shaanxi, China.
School of Water and Environment, Chang'an University, Xi'an 710064, Shaanxi, China.
Bioresour Technol. 2022 Jul;355:127271. doi: 10.1016/j.biortech.2022.127271. Epub 2022 May 5.
The inhibitory roles of hydroxylamine (NHOH) and hydrazine (NH) on nitrite-oxidizing bacteria were investigated in a comparative study. The results showed that nitrite accumulation was achieved by adding 5 mg-N/L NHOH or NH to two parallel sequencing batch reactors, with nitrite accumulation rate reaching 95.83% and 86.58% within 15 days after adopting aeration time control, respectively. Correspondingly, the maximum level of NO in typical cycles caused by NHOH addition was 0.18 mg-N/L, which was higher than obtained for NH. NHOH or NH showed strong inhibition on Nitrospira and promoted the enrichment of Nitrosomonas, with the effects of NHOH being more significant. However, nitritation began to deteriorate after the cessation of inhibitors addition. In conclusion, NHOH was a better inhibitor than NH for Nitrospira. The inhibitory role of NHOH was primarily related to NO toxicity, while for NH it was attributed to its own toxicity, with NO playing a smaller role.
本研究比较了羟胺（NHOH）和肼（NH）对亚硝酸盐氧化菌的抑制作用。结果表明，向两个平行序批式反应器中分别添加 5mg-N/L 的 NHOH 或 NH，通过控制曝气时间，15 天后即可实现亚硝酸盐积累，亚硝酸盐积累率分别达到 95.83%和 86.58%。相应地，添加 NHOH 引起的典型周期中 NO 的最大水平为 0.18mg-N/L，高于添加 NH 的情况。NHOH 或 NH 对 Nitrospira 具有很强的抑制作用，并促进了 Nitrosomonas 的富集，其中 NHOH 的作用更为显著。然而，停止添加抑制剂后，硝化作用开始恶化。总之，NHOH 是一种比 NH 更好的 Nitrospira 抑制剂。NHOH 的抑制作用主要与 NO 毒性有关，而 NH 的抑制作用则与其自身毒性有关，NO 的作用较小。
