Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, and Key Laboratory of Chinese Medicinal Resources Recycling Utilization, State Administration of Traditional Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
Phytother Res. 2020 Jul;34(7):1704-1720. doi: 10.1002/ptr.6652. Epub 2020 Mar 17.
Microcirculation, which connects macrocirculation and cells between arterioles and venules, plays a major role in the early onset of a variety of diseases. In this article, a dextran-induced microcirculation dysfunction (MCDF) model rats were adopted to evaluate the effects and mechanism of Salvia miltiorrhiza stem-leaf extracts based on plasma and urine metabonomics. The results showed the effective components of S. miltiorrhiza stem-leaf could significantly improve the hemorheology and coagulation index of MCDF rats and callback the expression of endothelin-1 (ET-1), induciblenitric oxide synthase (iNOS), vascularendothelial growth factor (VEGF), P-Selectin, thromboxane A2, 6-keto-PGF , TNF-α, and interleukin-1β to control group in MCDF rats. The decrease of microvessel density (MVD) in lung and thymus caused by MCDF was upgraded by Salvia miltiorrhiza stem-leaf. Based on the plasma and urine metabolic data, 20 potential biomarkers were identified. These biomarkers are mainly related to linoleic acid metabolism, glutathione metabolism, pantothenate and coenzyme A biosynthesis, pentose and glucuronate interconversions, pyruvate metabolism, glyoxylate and dicarboxylate metabolism, beta-alanine metabolism, and citrate cycle. The results indicated that the effective components of S. miltiorrhiza stem-leaf can improve the hemorheological disorder and vascular endothelial function. Meanwhile, the effective components can regulate potential biomarkers and correlated metabolic pathway, which can provide guidance for the research and development of new drugs for MCDF.
微循环连接着大循环和动静脉之间的细胞，在各种疾病的早期发生中起着重要作用。本文采用葡聚糖诱导的微循环功能障碍（MCDF）大鼠模型，基于血浆和尿液代谢组学评价丹参茎叶提取物的作用及机制。结果表明，丹参茎叶的有效成分可显著改善 MCDF 大鼠的血液流变学和凝血指标，回调 ET-1、诱导型一氧化氮合酶（iNOS）、血管内皮生长因子（VEGF）、P-选择素、血栓素 A2、6-酮-PGF1α、TNF-α和白细胞介素-1β在 MCDF 大鼠中的表达。MCDF 引起的肺和胸腺中小血管密度（MVD）降低，被丹参茎叶上调。基于血浆和尿液代谢数据，鉴定出 20 个潜在生物标志物。这些生物标志物主要与亚油酸代谢、谷胱甘肽代谢、泛酸和辅酶 A 生物合成、戊糖和葡萄糖醛酸转化、丙酮酸代谢、乙醛酸和二羧酸代谢、β-丙氨酸代谢以及柠檬酸循环有关。结果表明，丹参茎叶的有效成分可以改善血液流变学紊乱和血管内皮功能。同时，有效成分可以调节潜在的生物标志物和相关代谢途径，为 MCDF 的新药研究和开发提供指导。
