Zhou Tao, Xu Shunfu, Chen Xu, Zhang Nianzhi, Hu Die, Wang Wendong
Department of biochemistry and molecular biology, Anhui University of Chinese Medicine, Hefei 230012, China.
Department of respiratory medicine, the First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230031, China.
J Tradit Chin Med. 2018 Feb;38(1):61-66.
To observe the effect of Xiaochuanping powder (XP), a traditional Chinese prescription for the treatment of cough and asthma, on serum concentrations of eosinophil cationic protein (ECP), αtumor necrosis factor (TNF)-α;, and interleukin (IL)-4, eosinophil counts, as well as expression of matrix metalloproteinase (MMP)-9, tissue inhibitor of metalloproteinase (TIMP)-1 in the lung tissues of asthmatic rats.
Sixty clean-grade Sprague-Dawley rats were divided randomly into six groups: normal control (NC), asthma model, Guilong Kechuanning (GK) group, as well as high-, intermediate-, and low- dose XP groups. Rats were sensitized with ovalbumin (OVA) to trigger asthma. Serum concentrations of ECP, TNF-α; and IL-4, eosinophil counts, as well as expression of MMP-9 and TIMP-1 in lung tissues were evaluated using an immunofluorescence method. mRNA expression of MMP-9 and TIMP-1 was determined using real-time quantitative polymerase chain reaction.
Compared with the asthma-model group, serum concentrations of ECP, TNF-α, and IL-4, and eosinophil counts decreased significantly in the high- and intermediate-dose XP groups and GK group (all P < 0.01). Protein expression of MMP-9 and TIMP-1 decreased significantly in the high- and intermediate-dose XP groups and GK group (all P < 0.01). Transcription of MMP-9 and TIMP-1 mRNA and the ratio of expression of MMP-9: TIMP-1 in lung tissue were significantly lower (P < 0.01).
XP can reduce TNF-α secretion, suppress the infiltration / activation of eosinophils, reduce serum concentrations of ECP and IL-4, reduce the protein and mRNA expression of MMP-9 and TIMP-1 in lung tissue, and regulate the balance between expression of MMP-9 and TIMP-1. In these ways, XP alleviated the inflammation and remodeling of the airways.
观察治疗咳嗽和哮喘的中药方剂小川平散（XP）对哮喘大鼠血清嗜酸性粒细胞阳离子蛋白（ECP）、α肿瘤坏死因子（TNF）-α、白细胞介素（IL）-4浓度、嗜酸性粒细胞计数，以及肺组织中基质金属蛋白酶（MMP）-9、金属蛋白酶组织抑制剂（TIMP）-1表达的影响。
将60只清洁级Sprague-Dawley大鼠随机分为6组：正常对照组（NC）、哮喘模型组、桂龙咳喘宁（GK）组以及XP高、中、低剂量组。用卵清蛋白（OVA）致敏大鼠以诱发哮喘。采用免疫荧光法评估血清ECP、TNF-α和IL-4浓度、嗜酸性粒细胞计数，以及肺组织中MMP-9和TIMP-1的表达。采用实时定量聚合酶链反应测定MMP-9和TIMP-1的mRNA表达。
与哮喘模型组相比，XP高、中剂量组和GK组血清ECP、TNF-α和IL-4浓度以及嗜酸性粒细胞计数均显著降低（均P<0.01）。XP高、中剂量组和GK组MMP-9和TIMP-1蛋白表达均显著降低（均P<0.01）。肺组织中MMP-9和TIMP-1 mRNA转录及MMP-9:TIMP-1表达比值均显著降低（P<0.01）。
XP可减少TNF-α分泌，抑制嗜酸性粒细胞浸润/活化，降低血清ECP和IL-4浓度，降低肺组织中MMP-9和TIMP-1的蛋白及mRNA表达，并调节MMP-9和TIMP-1表达之间的平衡。通过这些方式，XP减轻了气道炎症和重塑。
