Encephalopathy of Internal Medicine of Trational Chinese Medicine, Changchun University of Chinese Medicine, Jilin 130117, China.
Department of Trational Chinese Medicine, the Second People's Hospital of Chengyang District of Qingdao, Qingdao 266000, China.
J Tradit Chin Med. 2019 Feb;39(1):81-88.
To investigate the effect of Qiangxin Huoli decoction on rats with chronic heart failure (CHF) induced by adriamycin (ADR), and to investigate the underlying mechanism of this effect.
Ninety-six healthy Wistar rats were divided into six groups: control, CHF model, CHF treated by Shenfu injection, and three CHF groups treated with Qiangxin Huoli decoction at high, medium, and low doses, respectively. Qiangxin Huoli decoction was administered orally to protect the stomach in the three Qiangxin Huoli decoction groups, while the control group and the CHF model group were administered the same volume of 0.9% physiological saline, and the Shenfu group wereadministered the same volume of Shenfu injection. Ten days later, the CHF model was then induced in all groups except the control group by intraperitoneal injection of ADR at gradient dose intervals. The bodyweights were recorded on days 10, 20, 30, and 40. Hemodynamic indices were recorded, including left ventricular systolic pressure (LVSP), left ventricular end-diastolic pressure (LVEDP), maximum increase in left ventricular pressure (+dp/dtmax), maximum decrease in left ventricular pressure (-dp/dtmax), heart rate (HR), and electrocardiogram using an eight-channel physiological recorder with LabChart software monitoring. The plasma brain natriuretic peptide (BNP) concentration was determined by enzyme-linked immunosorbent adsorption. The expressions of B-cell lymphoma-2 (Bcl-2) and Bcl-2-associated X protein (Bax) were detected by immunohistochemical methods.
The CHF model group were in poor condition, and the mean bodyweight was significantly decreased compared with the control group. Furthermore, compared with the control group, the CHF groups had significantly decreased LVSP, +dp/dtmax, and -dp/dtmax, and significantly increased LVEDP. The CHF groups also showed significant increases in HR, S-T segment elevation, and plasma BNP levels compared with the control group. Compared with the CHF model group, the treatment groups had significantly increased Bax expression (P < 0.05) and significantly decreased Bcl-2 expression (P < 0.01), indicating less apoptosis. The high dose Qiangxin Huoli decoction group and the Shenfu group showed the most significant improvements.
In the rat model of CHF, Qiangxin Huoli decoction significantly reduces the abnormal hemodynamics, improves cardiac function, reduces plasma BNP concentration, regulates the expression of apoptosis proteins, inhibits the apoptosis of myocardial cells, and plays a protective role.
探讨强心活利汤对阿霉素（ADR）诱导的慢性心力衰竭（CHF）大鼠的作用，并探讨其作用机制。
96 只健康 Wistar 大鼠随机分为 6 组：对照组、CHF 模型组、参附注射液治疗组和强心活利汤高、中、低剂量组，每组 16 只。参附注射液组和 3 个强心活利汤组给予参附注射液灌胃保护胃，对照组和 CHF 模型组给予等体积的 0.9%生理盐水，强心活利汤组给予等体积的强心活利汤。10 天后，除对照组外，其余各组均采用腹腔注射 ADR 诱导 CHF 模型，剂量梯度递增。记录第 10、20、30、40 天的体质量。采用八通道生理记录仪和 LabChart 软件监测心电图，记录左心室收缩压（LVSP）、左心室舒张末期压（LVEDP）、左心室压力最大上升速率（+dp/dtmax）、左心室压力最大下降速率（-dp/dtmax）、心率（HR）等血流动力学指标。采用酶联免疫吸附法测定血浆脑钠肽（BNP）浓度。采用免疫组织化学法检测 B 细胞淋巴瘤-2（Bcl-2）和 Bcl-2 相关 X 蛋白（Bax）的表达。
CHF 模型组大鼠一般情况较差，体质量明显低于对照组。与对照组相比，CHF 组 LVSP、+dp/dtmax、-dp/dtmax 明显降低，LVEDP 明显升高；HR、ST 段抬高、血浆 BNP 水平明显升高。与 CHF 模型组相比，各治疗组 Bax 表达明显增加（P<0.05），Bcl-2 表达明显减少（P<0.01），提示细胞凋亡减少。高剂量强心活利汤组和参附注射液组改善最明显。
在 CHF 大鼠模型中，强心活利汤可显著改善异常血流动力学，改善心功能，降低血浆 BNP 浓度，调节凋亡蛋白表达，抑制心肌细胞凋亡，发挥保护作用。
