Wang Chunli, Zhang Liang, Wu Meimei, Jing Yuntiao, Lai Xiaoping, Huang Song, Zhang Xiaojun
School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
J Tradit Chin Med. 2018 Aug;38(4):523-534.
To examine the antioxidative and hepatoprotective activities of the effective fraction separated from the fruit of Livistona chinensis (FLC) and to explore the possible mechanism.
The antioxidative activities of the various fractions separated from FLC were analyzed by in vitro DPPH (1,1-diphenyl-2-picrylhydrazyl radical 2, 2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl, DPPH) radical and superoxide anion free radical (O2- scavenging assays. The potential hepatoprotective effects of the ethyl acetate fraction separated from FLC (EFLC) were examined in LO2 cells and mice. LO2 cells were incubated with EFLC and exposed to H2O2-induced oxidative stress. BABL/C mice were orally administered EFLC for consecutive 7 d, and fulminant hepatitis was induced by cauda vein injection of ConA on day 7.
EFLC showed prominent antioxidative effects in DPPHï¼?and O2ï¼?scavenging assays. EFLC pretreatment effectively protected LO2 cells from H2O2-induced oxidative stress by decreasing apoptosis and raising the levels of antioxidant enzymes. Additionally, EFLC protected mice against ConA-induced fulminant hepatitis by markedly reducing the serum levels of alanine transaminase and aspartate aminotransferase, attenuating histological damage of the mouse liver, and elevating the levels of glutathione peroxidase and total superoxide dismutase, while decreasing the contents of methane dicarboxylic aldehyde, tumor necrosis factor-¦Á, and interleukin-1¦Â in the mouse liver. Furthermore, EFLC pretreatment downregulated the protein expression of B-cell lymphoma 2 (Bcl-2) associated X protein, caspase-3, caspase-8, Fas, and FasL, while upregulating the protein expression of Bcl-2 in the mouse liver.
These findings revealed that EFLC effectively protected against in vivo and in vitro liver injury, and the mechanism is closely associated with its antioxidative and anti-apoptotic properties.
研究从蒲葵果实中分离得到的有效部位的抗氧化及保肝活性，并探讨其可能机制。
通过体外DPPH（1,1-二苯基-2-苦基肼自由基、2,2-二苯基-1-（2,4,6-三硝基苯基）肼，DPPH）自由基和超氧阴离子自由基清除试验分析从蒲葵果实中分离得到的各部位的抗氧化活性。在LO2细胞和小鼠中检测从蒲葵果实中分离得到的乙酸乙酯部位（EFLC）的潜在保肝作用。将LO2细胞与EFLC共同孵育，并使其暴露于过氧化氢诱导的氧化应激环境中。BABL/C小鼠连续7天口服给予EFLC，第7天通过尾静脉注射刀豆蛋白A诱导爆发性肝炎。
EFLC在DPPH和超氧阴离子自由基清除试验中表现出显著的抗氧化作用。EFLC预处理通过减少细胞凋亡和提高抗氧化酶水平，有效保护LO2细胞免受过氧化氢诱导的氧化应激损伤。此外，EFLC通过显著降低血清丙氨酸转氨酶和天冬氨酸转氨酶水平、减轻小鼠肝脏组织学损伤、提高谷胱甘肽过氧化物酶和总超氧化物歧化酶水平，同时降低小鼠肝脏中丙二醛、肿瘤坏死因子-α和白细胞介素-1β的含量，保护小鼠免受刀豆蛋白A诱导的爆发性肝炎。此外，EFLC预处理下调小鼠肝脏中B细胞淋巴瘤-2（Bcl-2）相关X蛋白、半胱天冬酶-3、半胱天冬酶-8 Fas和FasL的蛋白表达，同时上调Bcl-2的蛋白表达。
这些研究结果表明，EFLC能有效保护体内和体外肝脏损伤，其机制与其抗氧化和抗凋亡特性密切相关。
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