School of Biosciences, Cardiff University, Cardiff, UK.
Chem Commun (Camb). 2020 Apr 21;56(31):4308-4311. doi: 10.1039/d0cc00596g. Epub 2020 Mar 18.
Metalloporphyrins play important roles in areas ranging from biology to nanoscience. Using computational design, we converted metalloporphyrin specificity of cytochrome b from iron to fluorogenic zinc. The new variant had a near total preference for zinc representing a switch in specificity, which greatly enhanced the negligible aqueous fluorescence of free ZnPP in vitro and in vivo.
金属卟啉在从生物学到纳米科学的各个领域都发挥着重要作用。我们通过计算设计，将细胞色素 b 中铁的金属卟啉特异性转化为荧光锌。这种新变体几乎完全偏爱锌，代表了特异性的转变，极大地增强了游离 ZnPP 在体外和体内的可忽略不计的水荧光。
