Alcala-Torano Rafael, Halloran Nicholas, Gwerder Noah, Sommer Dayn J, Ghirlanda Giovanna
School of Molecular Sciences, Arizona State University, Tempe, AZ, United States.
Front Mol Biosci. 2021 Apr 15;8:609654. doi: 10.3389/fmolb.2021.609654. eCollection 2021.
The current trend in atmospheric carbon dioxide concentrations is causing increasing concerns for its environmental impacts, and spurring the developments of sustainable methods to reduce CO to usable molecules. We report the light-driven CO reduction in water in mild conditions by artificial protein catalysts based on cytochrome and incorporating cobalt protoporphyrin IX as cofactor. Incorporation into the protein scaffolds enhances the intrinsic reactivity of the cobalt porphyrin toward proton reduction and CO generation. Mutations around the binding site modulate the activity of the enzyme, pointing to the possibility of further improving catalytic activity through rational design or directed evolution.
当前大气中二氧化碳浓度的趋势引发了人们对其环境影响的日益关注，并促使人们开发可持续方法将二氧化碳还原为可用分子。我们报告了在温和条件下，基于细胞色素并结合原卟啉钴IX作为辅因子的人工蛋白质催化剂在水中光驱动二氧化碳还原的情况。将其整合到蛋白质支架中可增强钴卟啉对质子还原和二氧化碳生成的固有反应性。结合位点周围的突变调节了酶的活性，这表明通过合理设计或定向进化进一步提高催化活性的可能性。
