Pazur J H, Miskiel F J, Liu B
Paul M. Althouse Laboratory, Pennsylvania State University, University Park 16802.
Anal Biochem. 1988 Oct;174(1):46-53. doi: 10.1016/0003-2697(88)90517-9.
Several types of oligosaccharides with glucose, arabinose, or galactose units have been prepared by chemical degradation of oligosaccharides of known structures and by enzymatic syntheses utilizing macerans amylase or yeast galactosyltransferase and appropriate substrates and cosubstrates. The ring forms of reducing units of these oligosaccharides and of oligosaccharides composed of glucose and mannose have been identified by a combined method of analysis based on methylation, gas-liquid chromatography, and mass spectrometry. In the dimethyl sulfoxide solvent used for the methylations, the oligosaccharides with arabinose or galactose units at the reducing ends occur as arabinofuranose or galactofuranose ring forms to the extent of 55 and 65%, respectively. The remainder of these monosaccharide units are present in the pyranose ring form. Oligosaccharides with glucose or mannose units at the reducing ends occur primarily in the pyranose ring form. An interesting observation is the finding that the reducing units which carry substituents linked by alpha-glycosidic linkages occur in a higher percentage in the furanose ring forms than those which carry substituents linked by beta-glycosidic linkages.
通过对已知结构的寡糖进行化学降解，以及利用短杆菌淀粉酶或酵母半乳糖基转移酶与合适的底物和共底物进行酶促合成，已制备出几种含有葡萄糖、阿拉伯糖或半乳糖单元的寡糖。这些寡糖以及由葡萄糖和甘露糖组成的寡糖的还原单元的环状形式，已通过基于甲基化、气液色谱和质谱的组合分析方法得以鉴定。在用于甲基化的二甲基亚砜溶剂中，还原端带有阿拉伯糖或半乳糖单元的寡糖分别以阿拉伯呋喃糖或半乳糖呋喃糖环状形式存在，比例分别为55%和65%。这些单糖单元的其余部分以吡喃糖环状形式存在。还原端带有葡萄糖或甘露糖单元的寡糖主要以吡喃糖环状形式存在。一个有趣的发现是，带有通过α-糖苷键连接的取代基的还原单元以呋喃糖环状形式存在的百分比高于带有通过β-糖苷键连接的取代基的还原单元。
