Kennedy J F, Chaplin M F
Biochem J. 1972 Nov;130(2):417-23. doi: 10.1042/bj1300417.
A preparation of human follicle-stimulating hormone has been subjected to methylation analysis by using the methyl sulphinyl carbanion-dimethyl sulphoxide-methyl iodide method. The hydrolysis products of the methylated glycoprotein were reduced, acetylated, analysed by gas-phase chromatography and mass spectrometry and identified by comparison with standards. Methylation analysis demonstrated that (1) the d-galactose, mannose, fucose and 2-amino-2-deoxyglucose units exist in the pyranose forms, (2) the 2-amino-2-deoxyglucose units are N-acetylated, (3) the fucopyranose units occupy terminal non-reducing positions, (4) the d-galactopyranose units are linked in the 1- and 2-positions, (5) the mannopyranose units exist in three forms, some as terminal non-reducing residues, some as 1,6-linked residues and some as 1,3,4-linked branch points, and (6) the 2-acetamido deoxyglucopyranose units are 1,6-linked. These structural assignments are compared with other data previously obtained for the carbohydrate moieties of follicle-stimulating hormone.
已使用亚磺酰甲基碳负离子-二甲基亚砜-甲基碘法对人卵泡刺激素制剂进行甲基化分析。将甲基化糖蛋白的水解产物进行还原、乙酰化，通过气相色谱和质谱分析，并与标准品比较进行鉴定。甲基化分析表明：（1）D-半乳糖、甘露糖、岩藻糖和2-氨基-2-脱氧葡萄糖单元以吡喃糖形式存在；（2）2-氨基-2-脱氧葡萄糖单元为N-乙酰化；（3）岩藻吡喃糖单元占据末端非还原位置；（4）D-半乳糖吡喃糖单元以1-位和2-位连接；（5）甘露吡喃糖单元存在三种形式，一些作为末端非还原残基，一些作为1,6-连接残基，一些作为1,3,4-连接分支点；（6）2-乙酰氨基脱氧吡喃葡萄糖单元为1,6-连接。将这些结构归属与先前获得的关于卵泡刺激素碳水化合物部分的其他数据进行比较。
