Guan H X, Xiao Y, Kan B, Zhou H J, Luo D, Shi C, Qian H M, Qian Y H
Wuxi Center for Disease Control and Prevention, Wuxi 214023, China.
State Key Laboratory for Infectious Disease Prevention and Control/National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2020 Mar 6;54(3):323-326. doi: 10.3760/cma.j.issn.0253-9624.2020.03.015.
To conduct outbreak identification and transmission factor analysis of typhoid epidemic occurred in Xinqiao town, Jiangyin city from June to September 2016. A total of 14 strains of Salmonella typhi isolated from confirmed cases were collected, and 65 external environment samples and 13 food samples related to the outbreak were taken. Real-time PCR was used to detect specific gene of Salmonella typhi in the samples. Conventional method was used to isolate strains. The strains isolated from both the samples and patients in the epidemic were subjected to antimicrobial susceptibility testing and PFGE molecular characteristics. Salmonella typhi strain was isolated from one external sample (well water of a deli processing plant). The results of drug susceptibility showed that 15 strains were resistant to nalidixic acid. A total of 15 strains of Salmonella typhi were divided into 2 molecular patterns by pulsed field gel electrophoresis. The fingerprints of PFGE from the 13 patients and the environmental isolate were completely consistent, and there was one band difference from the other patient isolate. Combined with the epidemiological investigation and laboratory test results, it was determined that the outbreak was caused by genetic clone of the same Salmonella typhi. Food processing plant should be one of the key links.
对2016年6月至9月在江阴市新桥镇发生的伤寒疫情进行暴发识别及传播因素分析。共采集确诊病例分离出的14株伤寒沙门菌，采集与疫情相关的65份外环境样本和13份食品样本。采用实时荧光定量PCR检测样本中伤寒沙门菌的特异基因，采用常规方法分离菌株。对疫情中样本及患者分离出的菌株进行药敏试验及PFGE分子特征分析。从1份外环境样本（一家熟食加工厂井水）中分离出伤寒沙门菌菌株。药敏结果显示15株对萘啶酸耐药。15株伤寒沙门菌经脉冲场凝胶电泳分为2种分子型别。13例患者及环境分离株的PFGE指纹图谱完全一致，与另1例患者分离株有1条带的差异。结合流行病学调查及实验室检测结果，确定此次暴发系由同一株伤寒沙门菌基因克隆引起，食品加工厂应是关键环节之一。
