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来自克罗地亚的一种非常罕见喀斯特蝰蛇 ssp. 的毒液的生物学活性和蛋白质组图谱。

Biological Activities and Proteomic Profile of the Venom of ssp., a very Rare Karst Viper from Croatia.

机构信息

University of Zagreb, Centre for Research and Knowledge Transfer in Biotechnology, Rockefellerova 10, HR-10 000 Zagreb, Croatia.

Jožef Stefan Institute, Department of Molecular and Biomedical Sciences, Jamova cesta 39, SI-1000 Ljubljana, Slovenia.

出版信息

Toxins (Basel). 2020 Mar 16;12(3):187. doi: 10.3390/toxins12030187.

DOI:10.3390/toxins12030187
PMID:32188060
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7150868/
Abstract

The karst viper ( ssp.) favours high-mountain dry grasslands in southern and south-eastern Croatia. It is medically less important than other species, because of its remote habitat and the very small amount of venom that it injects by its relatively short fangs. The scientific literature on deals mostly with the morphology, ecology and distribution range of this snake, due to the species' conservation issues, while the toxinological aspects of its venom have not so far been investigated. Here we report on the composition and biological activity of the ssp. venom. Using a proteomics approach, we have identified 25 proteins in the venom that belong to seven protein families: snake venom metalloproteinase, serine protease, secreted phospholipase A, cysteine-rich secretory protein, snake C-type lectin-like protein, serine protease inhibitor and nerve growth factor. The ssp. venom was found to be distinctively insecticidal. Its lethal toxicity towards crickets was more than five times greater than that of venom, while the opposite held in mice. Interestingly, the mode of dying after injecting a mouse with ssp. venom may suggest the presence of a neurotoxic component. Neurotoxic effects of European vipers have so far been ascribed exclusively to ammodytoxins and ammodytoxin-like basic secreted phospholipases A. Structural and immunological analyses of the ssp. venom, however, confirmed that ammodytoxin-like proteins are not present in this venom.

摘要

喀斯特蝰（ssp.）喜欢克罗地亚南部和东南部的高山干燥草原。由于其栖息地偏远，而且毒液注入量相对较短的毒牙也非常少，因此在医学上不如其他物种重要。关于该蛇种的科学文献主要涉及形态学、生态学和分布范围，这是由于该物种的保护问题，而其毒液的毒素学方面尚未得到研究。在这里，我们报告了喀斯特蝰（ssp.）毒液的组成和生物学活性。使用蛋白质组学方法，我们在毒液中鉴定出 25 种属于七个蛋白质家族的蛋白质：蛇毒金属蛋白酶、丝氨酸蛋白酶、分泌型磷脂酶 A、富含半胱氨酸的分泌蛋白、蛇 C 型凝集素样蛋白、丝氨酸蛋白酶抑制剂和神经生长因子。喀斯特蝰（ssp.）毒液具有明显的杀虫活性。它对蟋蟀的致死毒性比蝰蛇毒液高出五倍以上，而对老鼠则相反。有趣的是，用喀斯特蝰（ssp.）毒液注射老鼠后死亡的方式可能表明存在神经毒性成分。到目前为止，欧洲毒蛇的神经毒性作用仅归因于氨肽毒素和氨肽毒素样碱性分泌型磷脂酶 A。然而，对喀斯特蝰（ssp.）毒液的结构和免疫学分析证实，这种毒液中不存在氨肽毒素样蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/72c312a37814/toxins-12-00187-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/bd92e95ae985/toxins-12-00187-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/5808d553d0cf/toxins-12-00187-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/eae2fd89bd50/toxins-12-00187-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/80af1f12ce74/toxins-12-00187-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/72c312a37814/toxins-12-00187-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/bd92e95ae985/toxins-12-00187-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/5808d553d0cf/toxins-12-00187-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/eae2fd89bd50/toxins-12-00187-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/80af1f12ce74/toxins-12-00187-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f921/7150868/72c312a37814/toxins-12-00187-g005.jpg

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