Sami Ullah
Department of Botany, University of Peshawar, Peshawar 24420, Pakistan.
J Tradit Chin Med. 2017 Aug;37(4):444-451.
To evaluate the anticancer, antioxidant and antimicrobial activities along with total phenolic and flavonoids contents extracted from Lespedeza bicolor indigenous to Khyber Pukhtoonkhwa, Pakistan.
Anticancer activity against human lung carcinoma (LU-1) and human prostate carcinoma (LnCap), antimicrobial against Aspergillus fumigates, Aspergillus niger, Fusarium solani, Mucor sp Klebsiella pneumonia and Enterococcus and antioxidant activity on 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) and Hydrogen peroxide radicals scavenging activity were assessed in methanol extract of Lespedeza bicolor.
Lespedeza bicolor aerial parts and root extract showed IC50 value of 12.5 and 50 μg/mL against LU-1 whereas, ≤ 12.5 and 12 ug/mL were calculated against LnCap cell line. Minimum inhibition concentration (MIC) value of 20-35 ug/mL has been observed against Aspergillus fumigates, Aspergillus niger, Fusarium solani and Mucor sp in comparision with 1-2.5 ug/mL of Terbinafine used as a standard fungicide. MIC value of 20 and 35 ug/mL of Lespedeza bicolor aerial parts and root extract against bacterial pathogen Klebsiella pneumonia and 20-50 ug/mL against Enterococcus has been measured. DPPH radical scavenging activity of Lespedeza bicolor with IC50 values of ≤ 50 ug/mL and ≤ 200 ug/mL was observed whereas, hydrogen peroxide scavenging activity with IC50 values of ≤ 25 ug/mL for aerial parts and ≤50 ug/mL for the root extract of Lespedeza bicolor has been shown.
These data suggested that the methanolic extract of Lespedeza bicolor could be potential candidates for natural antioxidants and anticancer.
评估从巴基斯坦开伯尔-普赫图赫瓦省本土的胡枝子中提取的物质的抗癌、抗氧化和抗菌活性以及总酚和黄酮含量。
评估胡枝子甲醇提取物对人肺癌（LU-1）和人前列腺癌（LnCap）的抗癌活性、对烟曲霉、黑曲霉、茄镰刀菌、毛霉属、肺炎克雷伯菌和肠球菌的抗菌活性以及对1,1-二苯基-2-苦基肼（DPPH）和过氧化氢自由基的清除活性。
胡枝子地上部分和根部提取物对LU-1的IC50值分别为12.5和50μg/mL，而对LnCap细胞系的IC50值分别≤12.5和12μg/mL。与用作标准杀菌剂的特比萘芬的1-2.5μg/mL相比，观察到对烟曲霉、黑曲霉、茄镰刀菌和毛霉属的最低抑菌浓度（MIC）值为20-35μg/mL。已测得胡枝子地上部分和根部提取物对细菌病原体肺炎克雷伯菌的MIC值为20和35μg/mL，对肠球菌的MIC值为20-50μg/mL。观察到胡枝子的DPPH自由基清除活性，IC50值分别≤50μg/mL和≤200μg/mL，而其地上部分对过氧化氢的清除活性IC50值≤25μg/mL，根部提取物对过氧化氢的清除活性IC50值≤50μg/mL。
这些数据表明，胡枝子甲醇提取物可能是天然抗氧化剂和抗癌剂的潜在候选物。
