Institute of Public Health and Clinical Nutrition, School of Medicine, University of Eastern Finland, 70211, Kuopio, Finland.
Functional Foods Forum, University of Turku, 20014, Turku, Finland.
FEMS Microbiol Lett. 2020 Mar 1;367(6). doi: 10.1093/femsle/fnaa050.
Here, we examined whether glyphosate affects the microbiota of herbivores feeding on non-target plants. Colorado potato beetles (Leptinotarsa decemlineata) were reared on potato plants grown in pots containing untreated soil or soil treated with glyphosate-based herbicide (GBH). As per the manufacturer's safety recommendations, the GBH soil treatments were done 2 weeks prior to planting the potatoes. Later, 2-day-old larvae were introduced to the potato plants and then collected in two phases: fourth instar larvae and adults. The larvae's internal microbiota and the adults' intestinal microbiota were examined by 16S rRNA gene sequencing. The beetles' microbial composition was affected by the GBH treatment and the differences in microbial composition between the control and insects exposed to GBH were more pronounced in the adults. The GBH treatment increased the relative abundance of Agrobacterium in the larvae and the adults. This effect may be related to the tolerance of some Agrobacterium species to glyphosate or to glyphosate-mediated changes in potato plants. On the other hand, the relative abundances of Enterobacteriaceae, Rhodobacter, Rhizobium and Acidovorax in the adult beetles and Ochrobactrum in the larvae were reduced in GBH treatment. These results demonstrate that glyphosate can impact microbial communities associated with herbivores feeding on non-target crop plants.
在这里，我们研究了草甘膦是否会影响以非靶标植物为食的草食动物的微生物群落。我们在含有未处理土壤或用草甘膦基除草剂（GBH）处理过的土壤的花盆中种植马铃薯，然后用马铃薯饲养美洲马铃薯甲虫（Leptinotarsa decemlineata）。按照制造商的安全建议，在种植马铃薯前两周进行 GBH 土壤处理。然后，将 2 天大的幼虫引入马铃薯植物中，并在两个阶段进行收集：四龄幼虫和成虫。通过 16S rRNA 基因测序检测幼虫的内部微生物群落和成虫的肠道微生物群落。GBH 处理影响了甲虫的微生物组成，并且在暴露于 GBH 的昆虫和对照昆虫之间，微生物组成的差异在成虫中更为明显。GBH 处理增加了幼虫和成虫中根瘤菌的相对丰度。这种影响可能与某些根瘤菌对草甘膦的耐受性或草甘膦介导的马铃薯植物变化有关。另一方面，在 GBH 处理中，成虫中的肠杆菌科、红杆菌、根瘤菌和食酸菌以及幼虫中的 Ochrobactrum 的相对丰度降低。这些结果表明，草甘膦可以影响以非靶标作物植物为食的草食动物相关的微生物群落。
