College of Life Sciences, Nanjing Forestry University, Nanjing 210037, China.
College of Life Sciences, Nanjing Normal University, Nanjing 210042, China.
Ecotoxicol Environ Saf. 2024 Jul 15;280:116549. doi: 10.1016/j.ecoenv.2024.116549. Epub 2024 Jun 8.
Roundup®, a prominent glyphosate-based herbicide (GBH), holds a significant position in the global market. However, studies of its effects on aquatic invertebrates, including molluscs are limited. Pomacea canaliculata, a large freshwater snail naturally thrives in agricultural environments where GBH is extensively employed. Our investigation involved assessing the impact of two concentrations of GBH (at concentrations of 19.98 mg/L and 59.94 mg/L, corresponding to 6 mg/L and 18 mg/L glyphosate) during a 96 h exposure experiment on the intestinal bacterial composition and metabolites of P. canaliculata. Analysis of the 16 S rRNA gene demonstrated a notable reduction in the alpha diversity of intestinal bacteria due to GBH exposure. Higher GBH concentration caused a significant shift in the relative abundance of dominant bacteria, such as Bacteroides and Paludibacter. We employed widely-targeted metabolomics analysis to analyze alterations in the hepatopancreatic metabolic profile as a consequence of GBH exposure. The shifts in metabolites primarily affected lipid, amino acid, and glucose metabolism, resulting in compromised immune and adaptive capacities in P. canaliculata. These results suggested that exposure to varying GBH concentrations perpetuates adverse effects on intestinal and hepatopancreatic health of P. canaliculata. This study provides an understanding of the negative effects of GBH on P. canaliculata and may sheds light on its potential implications for other molluscs.
Roundup®，一种重要的草甘膦基除草剂（GBH），在全球市场中占有重要地位。然而，关于其对水生无脊椎动物（包括软体动物）影响的研究有限。Pomacea canaliculata 是一种大型淡水蜗牛，自然栖息在广泛使用 GBH 的农业环境中。我们的研究评估了两种浓度的 GBH（浓度分别为 19.98 mg/L 和 59.94 mg/L，相当于 6 mg/L 和 18 mg/L 的草甘膦）对 P. canaliculata 肠道细菌组成和代谢物的 96 h 暴露实验的影响。16S rRNA 基因分析表明，由于暴露于 GBH，肠道细菌的 alpha 多样性显著降低。较高的 GBH 浓度导致优势细菌（如 Bacteroides 和 Paludibacter）的相对丰度发生显著变化。我们采用广泛靶向的代谢组学分析来分析由于暴露于 GBH 而导致的肝胰腺代谢谱的变化。代谢物的变化主要影响脂质、氨基酸和葡萄糖代谢，导致 P. canaliculata 的免疫和适应能力受损。这些结果表明，暴露于不同浓度的 GBH 会对 P. canaliculata 的肠道和肝胰腺健康产生持久的不利影响。本研究了解了 GBH 对 P. canaliculata 的负面影响，并可能揭示其对其他软体动物的潜在影响。
