通过酵母基因组合成和诱导进化发现和基因分型基因组结构变异。

Discovering and genotyping genomic structural variations by yeast genome synthesis and inducible evolution.

机构信息

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, 300072 Tianjin, People's Republic of China.

Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, 300072 Tianjin, People's Republic of China.

出版信息

FEMS Yeast Res. 2020 Mar 1;20(2). doi: 10.1093/femsyr/foaa012.

DOI:10.1093/femsyr/foaa012

PMID:32188997

Abstract

Genomic structural variations (SVs) promote the evolution of Saccharomyces cerevisiae, and play an important role in phenotypic diversities. Yeast genomic structures can be remodeled by design and bottom-up synthesis. The synthesis of yeast genome creates novel copy number variations (CNVs) and SVs and develops new strategies to discover gene functions. Further, an inducible evolution system SCRaMbLE, consisted of 3,932 loxPsym sites, was incorporated on synthetic yeast genome. SCRaMbLE enables genomic rearrangements at will and rapidly generates chromosomal number variations, and massive SVs under customized conditions. The impacts of genetic variations on phenotypes can be revealed by genome analysis and chromosome restructuring. Yeast genome synthesis and SCRaMbLE provide a new research paradigm to explore the genotypic mechanisms of phenotype diversities, and can be used to improve biological traits and optimize industrial chassis.

摘要

基因组结构变异（SVs）促进了酿酒酵母的进化，并在表型多样性中发挥着重要作用。酵母基因组结构可以通过设计和自下而上的合成进行重塑。酵母基因组的合成会产生新的拷贝数变异（CNVs）和 SVs，并开发出发现基因功能的新策略。此外，一个由 3932 个 loxPsym 位点组成的诱导进化系统 SCRaMbLE 被整合到合成酵母基因组中。SCRaMbLE 可以随意进行基因组重排，并在定制条件下快速产生染色体数量变异和大量 SVs。通过基因组分析和染色体重构，可以揭示遗传变异对表型的影响。酵母基因组合成和 SCRaMbLE 为探索表型多样性的基因型机制提供了一个新的研究范例，可用于改善生物特性和优化工业底盘。