Department of Pediatric Dentistry, CES University, Medellín, Colombia.
Department Biomedical and Applied Science, Indiana University School of Dentistry, Indianapolis, IN, USA.
Clin Oral Investig. 2020 Sep;24(9):3237-3242. doi: 10.1007/s00784-020-03197-5. Epub 2020 Mar 18.
To evaluate total absorbance, planktonic growth, biofilm formation, viability, metabolic activity, and pH of Streptococcus mutans UA159 cultures when different dilutions of Stevia rebaudiana Bertoni were applied and to determine the minimum inhibitory concentration (MIC) and the minimum biofilm inhibitory concentration (MBIC) of Stevia on S. mutans.
The effects of different dilutions of Stevia (0-400 mg/ml) on S. mutans total growth, planktonic growth, biofilm formation, viability, metabolic activity, and pH during a 72-h growth period were evaluated in this in vitro study. A stock solution was prepared by mixing 10 ml of tryptic soy broth (TSB) supplemented with 1% sucrose (TSBS) and 4 g of Stevia.
S. mutans total growth and biofilm formation decreased with reduced concentrations of Stevia. Furthermore, the MIC was 25 mg/ml and the MBIC was 6.25 mg/ml. Complete eradication of S. mutans was not observed with any of the Stevia concentrations. Planktonic growth of S. mutans was not repressed by high concentrations of Stevia and most of the Stevia concentrations generated an increased pH.
Because Stevia reduces biofilm and acid production, Stevia can be considered a non-cariogenic sweetener.
This study confirms the anticariogenic effect of Stevia, like it has been previously reported, but more studies on the most effective concentration are needed, and in the present study, the minimum inhibitory concentration (MIC) and the minimum biofilm inhibitory concentration (MBIC) was determined in the presence of sucrose. Additionally, this is the first study to evaluate the effect of Stevia on S. mutans metabolic activity.
评估甜菊叶不同稀释度对变异链球菌 UA159 总吸光度、浮游生长、生物膜形成、活力、代谢活性和 pH 的影响，并确定甜菊叶对变异链球菌的最小抑菌浓度 (MIC) 和最小生物膜抑制浓度 (MBIC)。
在这项体外研究中，评估了不同稀释度（0-400mg/ml）的甜菊叶对变异链球菌总生长、浮游生长、生物膜形成、活力、代谢活性和 pH 的影响，在 72 小时的生长期间。通过混合 10ml 含有 1%蔗糖的胰蛋白酶大豆肉汤（TSB）（TSBS）和 4g 甜菊叶来制备储备溶液。
随着甜菊叶浓度的降低，变异链球菌的总生长和生物膜形成减少。此外，MIC 为 25mg/ml，MBIC 为 6.25mg/ml。用任何甜菊叶浓度都没有观察到变异链球菌的完全根除。高浓度的甜菊叶不会抑制变异链球菌的浮游生长，并且大多数甜菊叶浓度会产生升高的 pH 值。
因为甜菊叶可以减少生物膜和酸的产生，所以可以将甜菊叶视为一种非致龋性甜味剂。
这项研究证实了甜菊叶的抗龋作用，就像之前报道的那样，但需要更多关于最有效浓度的研究，在本研究中，在存在蔗糖的情况下确定了最小抑菌浓度（MIC）和最小生物膜抑制浓度（MBIC）。此外，这是第一项评估甜菊叶对变异链球菌代谢活性影响的研究。
