Guo Mingzhu, Yang Kuan, Zhou Zhifei, Chen Yujiang, Zhou Ziye, Chen Peng, Huang Ruizhe, Wang Xiaojing
Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Stomatology, Department of Pediatric Dentistry, School of Stomatology, The Fourth Military Medical University, Xi'an, China.
Department of Orthodontics, The Affiliated Hospital of Qingdao University, Qingdao, China.
Front Microbiol. 2023 Apr 5;14:1128668. doi: 10.3389/fmicb.2023.1128668. eCollection 2023.
is the most prevalent biofilm-forming pathogen in dental caries, while is often detected in the presence of .
We aimed to evaluate the anti-caries effect of stevioside in medium trypticase soy broth (TSB) with or without sucrose supplementation compared with the same sweetness sucrose and xylitol in a dual-species model of and , based on planktonic growth, crystal violet assay, acid production, biofilm structural imaging, confocal laser scanning microscopy, and RNA sequencing.
Our results showed that compared with sucrose, stevioside significantly inhibited planktonic growth and acid production, changed the structure of the mixed biofilm, and reduced the viability of biofilm and the production of extracellular polysaccharides in dual-species biofilm. Through RNA-seq, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway impact analysis showed that stevioside decreased sucrose metabolism and increased galactose and intracellular polysaccharide metabolism in , and decreased genes related to GPI-modified proteins and secreted aspartyl proteinase (SAP) family in . In contrast to xylitol, stevioside also inhibited the transformation of fungal morphology of , which did not form mycelia and thus had reduced pathogenicity. Stevioside revealed a superior suppression of dual-species biofilm formation compared to sucrose and a similar anti-caries effect with xylitol. However, sucrose supplementation diminished the suppression of stevioside on and .
Our study is the first to confirm that stevioside has anticariogenic effects on and in a dual-species biofilm. As a substitute for sucrose, it may help reduce the risk of developing dental caries.
是龋齿中最常见的生物膜形成病原体，而 在 存在的情况下经常被检测到。
我们旨在评估甜菊糖苷在添加或不添加蔗糖的胰蛋白胨大豆肉汤（TSB）培养基中的防龋效果，并在 和 的双物种模型中，将其与相同甜度的蔗糖和木糖醇进行比较，比较内容基于浮游生长、结晶紫测定、酸产生、生物膜结构成像、共聚焦激光扫描显微镜和RNA测序。
我们的结果表明，与蔗糖相比，甜菊糖苷显著抑制浮游生长和酸产生，改变混合生物膜的结构，并降低双物种生物膜中生物膜的活力和细胞外多糖的产生。通过RNA测序，京都基因与基因组百科全书（KEGG）通路影响分析表明，甜菊糖苷降低了 中的蔗糖代谢，增加了半乳糖和细胞内多糖代谢，并降低了 中与GPI修饰蛋白和分泌天冬氨酸蛋白酶（SAP）家族相关的基因。与木糖醇相比，甜菊糖苷还抑制了 的真菌形态转化， 不形成菌丝体，因此致病性降低。与蔗糖相比，甜菊糖苷对双物种生物膜形成的抑制作用更强，与木糖醇具有相似的防龋效果。然而，添加蔗糖会减弱甜菊糖苷对 和 的抑制作用。
我们的研究首次证实甜菊糖苷在双物种生物膜中对 和 具有防龋作用。作为蔗糖的替代品，它可能有助于降低患龋齿的风险。
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