Verstijnen C P, Arends J W, Moerkerk P, Schutte B, van der Linden E, Kuypers-Engelen B, Bosman F T
Department of Pathology, State University Limburg, Maastricht, the Netherlands.
Anticancer Res. 1988 Nov-Dec;8(6):1193-200.
In a series of 61 primary colorectal carcinomas, we attempted to determine which primary tumor characteristics correlated with the possibility to continuously maintain tumor cells in vitro or in vivo, and to what extent the characteristics of a primary tumor were maintained in vitro or as xenograft. Four continuous cell lines and 10 serially transplantable tumors were obtained. Only one cell line could be maintained both in vitro and in vivo. Tumors that had metastized and tumors in the proximal colon showed a higher success for in vivo and in vitro growth. DNA analysis showed that in most xenografts the DNA index was identical to that the primary tumor. However, in some cases tumor cell subpopulations were lost or genetically variant new subpopulations were generated. In general, the degree of differentiation in the xenografts corresponded with the least differentiated areas in the primary tumor. Xenografts appeared to display comparable of antigen expression.
在一系列61例原发性结直肠癌中，我们试图确定哪些原发性肿瘤特征与在体外或体内持续维持肿瘤细胞的可能性相关，以及原发性肿瘤的特征在体外或作为异种移植时能在多大程度上得以保留。我们获得了4个连续传代的细胞系和10个可连续传代移植的肿瘤。只有一个细胞系能够在体外和体内都得以维持。发生转移的肿瘤以及近端结肠的肿瘤在体内和体外生长方面显示出更高的成功率。DNA分析表明，在大多数异种移植中，DNA指数与原发性肿瘤相同。然而，在某些情况下，肿瘤细胞亚群会丢失，或者会产生基因变异的新亚群。一般来说，异种移植中的分化程度与原发性肿瘤中分化最差的区域相对应。异种移植似乎显示出相当的抗原表达。
