Patil Chitra Laxmikant, Gaikwad Rajesh Prabhakar
Department of Periodontology, Government Dental College and Hospital, Mumbai, Maharashtra, India.
J Indian Soc Periodontol. 2020 Mar-Apr;24(2):150-155. doi: 10.4103/jisp.jisp_136_19. Epub 2020 Jan 27.
The present study aims to assess area of open dentinal tubules by use of diode laser and electrode alone and in combination with hydroxyapatite powder and strontium chloride powder by using the scanning electron microscope (SEM).
A double-blinded study was carried out with 30 extracted teeth with no carious lesion or restored teeth were selected and grouped randomly as Group A - diode laser, Group B - electrode application, Group C - hydroxyapatite powder plus diode laser, Group D - hydroxyapatite powder plus electrode application, Group E - strontium chloride powder plus diode laser, and Group F - strontium chloride powder plus electrode application. All the samples were made by preparing a notch of 2 mm measured with UNC-15 probe at cementoenamel junction using a diamond cylindrical bur at high speed. The teeth were then washed with distilled water and stored in solution containing 2.5% glutaraldehyde solution in 0.1 M sodium phosphate buffer for 24 h. The samples were treated as mentioned in above said groups and were viewed under SEM the degree of occlusion of the dentinal tubules were quantified using an image analyzer.
Highest number of open dentinal tubules was found with Group B, that is, electrode application, whereas Group C, that is, hydroxyapatite powder plus diode laser showed highest occluded tubules. One-way ANOVA showed statistically significant difference across all the groups ( = 0.000); further, intergroup comparison with Tukey's test showed better tubular occlusion with Group C.
The SEM results showed highest tubular occlusion with hydroxyapatite powder plus diode laser and least with electrode.
本研究旨在通过扫描电子显微镜(SEM),单独使用二极管激光和电极,以及将它们与羟基磷灰石粉末和氯化锶粉末联合使用,来评估开放牙本质小管的面积。
进行一项双盲研究,选取30颗无龋损或未修复的离体牙,随机分组为A组——二极管激光组、B组——电极应用组、C组——羟基磷灰石粉末加二极管激光组、D组——羟基磷灰石粉末加电极应用组、E组——氯化锶粉末加二极管激光组、F组——氯化锶粉末加电极应用组。所有样本均通过使用高速旋转的金刚石圆柱形车针,在牙釉质牙骨质界处制备一个用UNC - 15探针测量为2毫米的切口。然后用蒸馏水冲洗牙齿,并将其储存在含有2.5%戊二醛溶液的0.1 M磷酸钠缓冲液中24小时。样本按照上述分组进行处理,并在SEM下观察,使用图像分析仪对牙本质小管的封闭程度进行量化。
发现B组(即电极应用组)开放牙本质小管数量最多,而C组(即羟基磷灰石粉末加二极管激光组)显示封闭小管数量最多。单因素方差分析显示所有组之间存在统计学显著差异( = 0.000);此外,使用Tukey检验进行组间比较显示,C组的小管封闭效果更好。
SEM结果显示,羟基磷灰石粉末加二极管激光组的小管封闭效果最佳,电极组最差。