铒钇铝石榴石激光、二极管激光和诺瓦敏技术用于牙本质小管封闭的比较评估：一项体外扫描电子显微镜（SEM）和能量色散X射线分析（EDX）研究
Comparative Evaluation of Er: YAG Laser, Diode Laser, and Novamin Technology for Dentinal Tubule Occlusion: An In-Vitro Scanning Electron Microscope (SEM) and Energy Dispersive X-Ray Analysis (EDX) Study.
作者信息
Srivastava Vivek, Haldar Shreya, Srivastava Vipul, Meenawat Ajita, Shahab Khan Yasir, Huidrom Elizabeth
机构信息
Department of Periodontology, Sardar Patel Post Graduate Institute of Dental and Medical Sciences, Lucknow, IND.
Department of Conservative Dentistry and Endodontics, 32 Pearls Dental Clinic, Lucknow, IND.
出版信息
Cureus. 2024 Apr 23;16(4):e58806. doi: 10.7759/cureus.58806. eCollection 2024 Apr.
BACKGROUND
Dentinal hypersensitivity is a brief and painful oral condition that is characterized by an abrupt shooting sensation. Stimulation occurs when hot, cold, sweet, or sour food comes into contact with exposed dentinal tubules. The present study used a scanning electron microscope (SEM) and energy dispersive X-ray analysis (EDX) to investigate the efficacy of Er: YAG, 810 nm diode LASER, and NovaMin Technology in obstructing dentinal tubules.
MATERIAL AND METHODS
We extracted the outer layers of 30 human teeth to expose the tubules and then treated the surfaces with 17% ethylenediaminetetraacetic acid (EDTA) to create an etched effect. Three cohorts were created from the portions. Group A was subjected to the application of Erbium:Yttrium-Aluminum-Garnet (Er: YAG) laser with a power output of 2W in the non-contact mode for 1 minute. Group B was subjected to the application of an 810nm diode laser with a power output of 1W in continuous mode for 30 seconds. Group C was subjected to the application of NovaMin paste, which contains a 927 ppm fluoride content. Following the therapy, occluded dentinal tubules were analyzed using scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) for both quantitative and qualitative examination. The data analysis was conducted using a one-way analysis of variance (ANOVA) and Tukey's test, with a significance threshold of 0.05.
RESULTS
The average percentages of complete blockage of dentinal tubules in Groups A, B, and C were evaluated using the number of entirely unobstructed dentinal tubules at magnifications of 2000X (F = 3.05, p = 0.064), 5000X (F = 5.33, p = 0.011), and 10000X (F = 8.63, p = 0.001). The count of partially open dentinal tubules seen at magnifications of 2000X, 5000X, and 10000X was F = 10.15 (P < 0.001), F = 5.97 (p = 0.007), and F = 2.12 (p = 0.140) accordingly.
CONCLUSION
NovaMin technology has demonstrated more effectiveness in blocking dentinal tubules compared to 810nm diodes and Er: YAG lasers.
背景
牙本质过敏是一种短暂而疼痛的口腔疾病，其特征为突然的刺痛感。当热、冷、甜或酸的食物接触到暴露的牙本质小管时就会引发刺激。本研究使用扫描电子显微镜（SEM）和能量色散X射线分析（EDX）来研究铒激光（Er:YAG）、810纳米二极管激光和诺瓦敏技术封堵牙本质小管的效果。
材料与方法
我们提取了30颗人类牙齿的外层以暴露小管，然后用17%的乙二胺四乙酸（EDTA）处理表面以产生蚀刻效果。从这些部分创建了三个队列。A组在非接触模式下使用输出功率为2W的铒钇铝石榴石（Er:YAG）激光照射1分钟。B组在连续模式下使用输出功率为1W的810纳米二极管激光照射30秒。C组使用含有927 ppm氟含量的诺瓦敏糊剂。治疗后，使用扫描电子显微镜（SEM）和能量色散X射线光谱（EDX）对封闭的牙本质小管进行定量和定性分析。数据分析采用单因素方差分析（ANOVA）和图基检验，显著性阈值为0.05。
结果
使用在2000倍（F = 3.05，p = 0.064）、5000倍（F = 5.33，p = 0.011）和10000倍（F = 8.63，p = 0.001）放大倍数下完全未阻塞的牙本质小管数量，评估A、B和C组牙本质小管完全阻塞的平均百分比。在2000倍、5000倍和10000倍放大倍数下观察到的部分开放牙本质小管数量分别为F = 10.15（P < 0.001）、F = 5.97（p = 0.007）和F = 2.12（p = 0.140）。
结论
与810纳米二极管激光和Er:YAG激光相比，诺瓦敏技术在封堵牙本质小管方面显示出更高的有效性。
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