Fluminense Federal University-UFF, Niterói, Brazil.
Rural Federal University of Rio de Janeiro-UFRRJ, Seropédica, Brazil.
Reprod Domest Anim. 2020 Jun;55(6):747-752. doi: 10.1111/rda.13676. Epub 2020 Apr 17.
The aim of this study was to evaluate whether the RI and PI values would help in choosing the best embryo recipient, and observe whether CL vascularization would influence P4 production. During the breeding season 2018/2019, the study was conducted using 35 mares, which is used for reference to collect data for the project on the day of embryo transfer. The utilized mares were divided into five groups followed by the day after ovulation, with D0 being the day of ovulation. Therefore, the five groups are as follows: D4-mares that were on the 4th post-ovulation day; D5-mares that were on the 5th post-ovulation day; and doing so successively for D6, D7 and D8. On the day of embryo transfer, the CL of the mares that selected as recipients was evaluated by B-mode and power flow mode ultrasonography and the right and left dorsal branches of the uterine arteries by spectral Doppler ultrasonography. Blood samples were taken on the day of the embryo transfer for a dosage of P4 concentration by radioimmunoassay. No statistical difference was found between the variables when the mares were separated into pregnant and non-pregnant mares, or when they were separated by age groups. When the groups of mares were compared by the day of embryo transfer, the statistical difference was found between the groups D5 × D6 (p = .0053) and D6 × D8 (p = .0036) in RI variable. In PI variable, the statistical difference was found between the groups D4 × D8 (p = .049), D5 × D6 (p = .0446) and D6 × D8 (p = .0024). We conclude that the mares with RI measurement of uterine arteries near 1.0 are correlated to mares with high CL vascularization and elevated P4 concentration.
本研究旨在评估 RI 和 PI 值是否有助于选择最佳胚胎受体，并观察 CL 血管化是否会影响 P4 的产生。在 2018/2019 年繁殖季节，该研究使用了 35 匹母马，这些母马在胚胎移植当天被用于收集项目数据。将所使用的母马分为五组，然后是排卵后第 1 天（D0），即：D4-排卵后第 4 天的母马；D5-排卵后第 5 天的母马；D6、D7 和 D8 依次类推。在胚胎移植当天，通过 B 型和功率血流模式超声评估母马的 CL，并通过频谱多普勒超声评估子宫动脉的右侧和左侧背侧分支。胚胎移植当天采集母马的血液样本，通过放射免疫法测定 P4 浓度。当将母马分为怀孕和未怀孕母马或按年龄组分组时，变量之间没有发现统计学差异。当通过胚胎移植日期比较母马组时，发现 RI 变量中 D5×D6 组（p=0.0053）和 D6×D8 组（p=0.0036）之间存在统计学差异。在 PI 变量中，发现 D4×D8 组（p=0.049）、D5×D6 组（p=0.0446）和 D6×D8 组（p=0.0024）之间存在统计学差异。我们得出结论，子宫动脉 RI 测量值接近 1.0 的母马与 CL 血管化程度高和 P4 浓度升高的母马相关。
