USP - Universidade de São Paulo, Avenida Duque de Caxias Norte 225, Jardim Elite, Pirassununga, SP, Brazil.
M.V. Haras Laglória - Médico Veterinário responsável pelo Haras Laglória, Fazenda Salgada, 00000 - RT-19, Itajara-Itaperuna, RJ, Brazil.
Theriogenology. 2023 Nov;211:142-150. doi: 10.1016/j.theriogenology.2023.08.012. Epub 2023 Aug 18.
We aimed to evaluate the impact of corpus luteum (CL) and uterine characteristics accessed by B-mode and Color-Doppler ultrasonography in recipient mares at the time of embryo transfer (ET) on pregnancy outcomes. Recipient mares (n = 110), between days 3-9 after spontaneous ovulation, received a fresh embryo. Immediately before ET, the reproductive system was assessed by transrectal palpation for the following parameters: uterine tone (0-3), CL echogenicity (0-6), CL type (homogeneous, trabecular or anechoic center), luteal area (cm), uterine echogenicity (0-3), uterine edema (0-3), luteal blood perfusion (0-100%) and uterine blood perfusion (1-4). Additionally, a blood sample was collected by puncture of the jugular vein for plasma P4 dosage. Retrospectively, recipients were classified according to the luteal area (small [≤ 6 cm] or large [> 6 cm]), luteal blood perfusion (low [≤ 55%] or high [> 55%]), and plasma concentration of P4 (low ≤ 9.98 ng/mL or high > 9.98 ng/mL). Pregnancy diagnosis was performed at 12 and 30 days of gestation. Luteal blood perfusion was significantly higher (P = 0.04) in pregnant recipients (n = 83) than in non-pregnant recipients (n = 27). Overall P/ET was higher (P ≤ 0.02) in mares with high luteal blood perfusion and high P4. Luteal blood perfusion was the most adequate significant (P = 0.01) predictor of pregnancy compared with the luteal area and plasma P4 concentration. Only luteal blood perfusion showed a linear (P = 0.03) and cubic (P = 0.004) effect on P/ET. In conclusion, CL blood perfusion determined by color-Doppler can be used in real-time to select recipients with the greatest chance of maintaining pregnancy in equine ET programs.
我们旨在评估在胚胎移植 (ET) 时通过 B 型和彩色多普勒超声检查评估受体母马黄体 (CL) 和子宫特征对妊娠结局的影响。受体母马（n=110）在自然排卵后第 3-9 天接受新鲜胚胎。在 ET 前，通过直肠触诊评估生殖系统以下参数：子宫张力（0-3）、CL 回声（0-6）、CL 类型（均匀、小梁或无回声中心）、黄体面积（cm）、子宫回声（0-3）、子宫水肿（0-3）、黄体血流灌注（0-100%）和子宫血流灌注（1-4）。此外，通过颈静脉穿刺采集血液样本以测定血浆 P4 剂量。回顾性地，根据黄体面积（小 [≤6cm] 或大 [>6cm]）、黄体血流灌注（低 [≤55%] 或高 [>55%]）和血浆 P4 浓度（低 [≤9.98ng/mL] 或高 [>9.98ng/mL]）对受体进行分类。在妊娠和 30 天进行妊娠诊断。与未妊娠受体（n=27）相比，妊娠受体（n=83）的黄体血流灌注显著更高（P=0.04）。黄体血流灌注高且 P4 高的母马的总 P/ET 更高（P≤0.02）。与黄体面积和血浆 P4 浓度相比，黄体血流灌注是妊娠的最适宜的显著预测因子（P=0.01）。只有黄体血流灌注对 P/ET 表现出线性（P=0.03）和立方（P=0.004）的影响。总之，彩色多普勒确定的 CL 血流灌注可实时用于选择在马的 ET 方案中维持妊娠的可能性最大的受体。
