健康巴西女性队列产后人乳寡糖谱的变化。

Human Milk Oligosaccharide Profile Variation Throughout Postpartum in Healthy Women in a Brazilian Cohort.

机构信息

Nutritional Epidemiology Observatory, Department of Social and Applied Nutrition, Institute of Nutrition Josué de Castro, Rio de Janeiro Federal University, Rio de Janeiro 21941-590, Brazil.

Department of Pediatrics and Mother-Milk-Infant Center of Research Excellence, University of California, San Diego, La Jolla, CA 92161, USA.

出版信息

Nutrients. 2020 Mar 17;12(3):790. doi: 10.3390/nu12030790.

Human milk oligosaccharide (HMO) composition varies throughout lactation and can be influenced by maternal characteristics. This study describes HMO variation up to three months postpartum and explores the influences of maternal sociodemographic and anthropometric characteristics in a Brazilian prospective cohort. We followed 101 subjects from 28-35 gestational weeks (baseline) and throughout lactation at 2-8 (visit 1), 28-50 (visit 2) and 88-119 days postpartum (visit 3). Milk samples were collected at visits 1, 2 and 3, and 19 HMOs were quantified usinghigh-performance liquid chromatography with fluorescence detection (HPLC-FL). Friedman post-hoc test, Spearman rank correlation for maternal characteristics and HMOs and non-negative matrix factorization (NMF) were used to define the HMO profile. Most women were secretors (89.1%) and presented high proportion of 2'-fucosyllactose (2ꞌFL) at all three sample times, while lacto-N-tetraose (LNT, 2-8 days) and lacto-N-fucopentaose II (LNFPII, 28-50 and 88-119 days) were the most abundant HMOs in non-secretor women. Over the course of lactation, total HMO weight concentrations (g/L) decreased, but total HMO molar concentrations (mmol/L) increased, highlighting differential changes in HMO composition over time. In addition, maternal pre-pregnancy body mass index (BMI) and parity influence the HMO composition in healthy women in this Brazilian cohort.

人乳寡糖（HMO）的组成在哺乳期会发生变化，并且可能受到产妇特征的影响。本研究描述了产后三个月内 HMO 的变化，并在巴西前瞻性队列中探讨了产妇社会人口学和人体测量特征的影响。我们随访了 101 名 28-35 孕周（基线）的受试者，并在哺乳期的 2-8 天（第 1 次访视）、28-50 天（第 2 次访视）和 88-119 天（第 3 次访视）进行随访。在第 1、2 和 3 次访视时采集了奶样，并使用高效液相色谱-荧光检测法（HPLC-FL）定量分析了 19 种 HMO。采用 Friedman 事后检验、Spearman 秩相关分析产妇特征与 HMO 以及非负矩阵分解（NMF）来定义 HMO 图谱。大多数女性为分泌型（89.1%），在所有三个样本时间均具有较高的 2'-岩藻糖基乳糖（2'-FL）比例，而乳-N-四糖（LNT，2-8 天）和乳-N-岩藻五糖 II（LNFPII，28-50 天和 88-119 天）是在非分泌型女性中最丰富的 HMO。在哺乳期过程中，总 HMO 重量浓度（g/L）降低，但总 HMO 摩尔浓度（mmol/L）增加，突出了 HMO 组成随时间的差异变化。此外，在本巴西队列中，产妇妊娠前的体重指数（BMI）和产次影响健康女性的 HMO 组成。