从医院获得性感染分离出的大肠杆菌中，mar、sox和rob调控子对浓度梯度碳青霉烯应激的转录反应。
Transcriptional response of mar, sox and rob regulon against concentration gradient carbapenem stress within Escherichia coli isolated from hospital acquired infection.
作者信息
Chetri Shiela, Das Bhaskar Jyoti, Bhowmik Deepshikha, Chanda Debadatta Dhar, Chakravarty Atanu, Bhattacharjee Amitabha
机构信息
Assam University, Silchar, India.
Silchar Medical College and Hospital, Silchar, India.
出版信息
BMC Res Notes. 2020 Mar 19;13(1):168. doi: 10.1186/s13104-020-04999-2.
OBJECTIVE
The present study was carried out to investigate the transcriptional response of marA (Multiple antibiotic resistance A gene), soxS (Superoxide S gene) and rob (Right-origin-binding gene) under carbapenem stress.
RESULTS
12 isolates were found over-expressing AcrAB-TolC efflux pump system and showed reduced expression of OmpF (Outer membrane porin) gene were selected for further study. Among them, over expression of marA and rob was observed in 7 isolates. Increasing pattern of expression of marA and rob against meropenem was observed. The clones of marA and rob showed reduced susceptibility towards carbapenems.
目的
本研究旨在调查碳青霉烯压力下marA（多重抗生素耐药A基因）、soxS（超氧化物S基因）和rob（右起源结合基因）的转录反应。
结果
发现12株分离菌过表达AcrAB-TolC外排泵系统，且OmpF（外膜孔蛋白）基因表达降低，从中选择进行进一步研究。其中，7株分离菌中观察到marA和rob过表达。观察到marA和rob对美罗培南的表达呈增加模式。marA和rob的克隆对碳青霉烯类药物的敏感性降低。
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