Uchino J, Tsuburaya T, Kumagai F, Hase T, Hamada T, Komai T, Funatsu A, Hashimura E, Nakamura K, Kon T
First Department of Surgery, Hokkaido University School of Medicine, Tokushima University, Sapporo, Japan.
ASAIO Trans. 1988 Oct-Dec;34(4):972-7.
Monolayer cultures of hepatocytes were shown to have good function when compared with suspended cells. The authors manufactured a new hybrid artificial liver containing hepatocyte monolayers and evaluated its function. Hepatocytes isolated from an adult dog liver were cultured on collagen coated borosilicated glass (10 X 20 X 0.04 cm). A long-stroke artificial liver module was constructed by stacking 200 glass plates bearing hepatocytes, which were viable and functioned well during 4 weeks in perfusion culture; glyconeogenesis = 110 ng/micrograms DNA/min, urea synthesis = 3.6 ng/micrograms DNA/min and albumin synthesis = 29 micrograms/10(6) cells/day at the 5th day of perfusion. The levels were maintained for 2 weeks. The new device was applied to anhepatic dogs (Group 3) and compared with untreated (Group 1) and plasma exchange dogs (Group 2). The survival times were 21.3 +/- 5.6 hours in Group 1 (N = 6), 27.8 +/- 4.0 hours in Group 2 (N = 3), and 55.0 +/- 10.3 hours in Group 3 (N = 4). The longest survival was 65 hours. Serum ammonia increased to over 2,000 micrograms/dl after 12 hours in Groups 1 and 2, but remained under 400 micrograms/dl in Group 3. This new type of hybrid system may be a pilot design for the complete artificial liver.
与悬浮细胞相比，单层培养的肝细胞显示出良好的功能。作者制造了一种包含肝细胞单层的新型混合人工肝，并评估了其功能。从成年犬肝脏分离的肝细胞在胶原包被的硼硅酸盐玻璃（10×20×0.04厘米）上培养。通过堆叠200块带有肝细胞的玻璃板构建了一个长冲程人工肝模块，这些肝细胞在灌注培养的4周内保持存活且功能良好；在灌注第5天时，糖原异生为110纳克/微克DNA/分钟，尿素合成量为3.6纳克/微克DNA/分钟，白蛋白合成量为29微克/10⁶细胞/天。这些水平维持了2周。将该新装置应用于无肝犬（第3组），并与未治疗的犬（第1组）和进行血浆置换的犬（第2组）进行比较。第1组（N = 6）的存活时间为21.3±5.6小时，第2组（N = 3）为27.8±4.0小时，第3组（N = 4）为55.0±10.3小时。最长存活时间为65小时。第1组和第2组在12小时后血清氨升至超过2000微克/分升，但第3组保持在400微克/分升以下。这种新型混合系统可能是全人工肝的初步设计。
