Taguchi K, Matsushita M, Takahashi M, Uchino J
First Department of Surgery, Hokkaido University, School of Medicine, Sapporo, Japan.
Artif Organs. 1996 Feb;20(2):178-85. doi: 10.1111/j.1525-1594.1996.tb00724.x.
We have been developing a multiplate type of bioartificial liver (BAL) using primary cultured hepatocyte monolayers since 1987. This BAL has been shown to prolong the survival time of anhepatic dogs and rabbits. Initially, hepatocytes were cultivated on collagen-coated plates. To increase the multiplated BAL function, a sandwich cultivation method was employed in which rat hepatocytes were cultured between two collagen gel layers and were then evaluated in both stationary and perfusion cultures. In the stationary culture, the sandwich method showed a higher activity in urea synthesis than in the other culture methods (culture on a collagen coating, culture on a collagen gel, and culture between a collagen coating and a collagen gel) for 14 days. In the perfusion culture, a BAL housing cultured hepatocytes (6.5 x 10(7) cells) in the sandwich system showed urea synthesis activity ranging from 17.5 to 22.6 micrograms/2 x 10(6) cells/90 min. This activity was maintained for 5 days in the perfusion culture. The sandwich type of cultivation is applicable to the multiplated BAL.
自1987年以来，我们一直在研发一种使用原代培养肝细胞单层的多板型生物人工肝（BAL）。已证明这种BAL可延长无肝犬和兔的存活时间。最初，肝细胞在胶原包被的平板上培养。为了增强多板型BAL的功能，采用了夹心培养法，即将大鼠肝细胞培养在两层胶原凝胶层之间，然后在静态培养和灌注培养中进行评估。在静态培养中，夹心培养法在14天内的尿素合成活性高于其他培养方法（胶原包被上培养、胶原凝胶上培养以及胶原包被和胶原凝胶之间培养）。在灌注培养中，夹心系统中容纳培养肝细胞（6.5×10⁷个细胞）的BAL显示尿素合成活性为17.5至22.6微克/2×10⁶个细胞/90分钟。这种活性在灌注培养中维持了5天。夹心培养类型适用于多板型BAL。
