Arias Daniel E, Veluchamy Chitraichamy, Dunfield Kari E, Habash Marc B, Gilroyed Brandon H
School of Environmental Sciences, University of Guelph Ridgetown Campus, Ridgetown N0P 2C0, Canada.
Centre for Agricultural Renewable Energy and Sustainability, University of Guelph Ridgetown Campus, Ridgetown N0P 2C0, Canada.
Bioresour Technol. 2020 Jun;306:123168. doi: 10.1016/j.biortech.2020.123168. Epub 2020 Mar 10.
This study assessed the effect of different swine manure (SM)/corn stover (CS) mixtures based on total solids (TS) content with respect to hygienization, microbial community dynamics and methane yields on batch anaerobic co-digestion performance. Different ratios of SM and CS with TS content between 0.69 and 6% digested at 75 d revealed SM had the greatest methane yield at 403.9 mL g volatile solids added (VS) and 86.31% VS reduction. BIOLOG AN microplates and lignocellulolytic enzyme assays proved to be rapid tools for characterizing microbial community metabolism as noted by the different carbon source utilization patterns between TS loadings. Hygienization of fecal indicator bacteria groups was achieved with some (E. coli) but not all groups (Clostridia spp.). The results showed that colorimetric biochemical assays and culture-based techniques can rapidly assess microbial community dynamics during co-digestion, and that TS- in the form of lignocellulosic biomass- influences microbial metabolic activities.
本研究评估了基于总固体（TS）含量的不同猪粪（SM）/玉米秸秆（CS）混合物对分批厌氧共消化性能的卫生化、微生物群落动态和甲烷产量的影响。在75天内消化的TS含量为0.69%至6%的不同比例的SM和CS表明，SM在添加403.9 mL挥发性固体（VS）时甲烷产量最高，VS减少了86.31%。BIOLOG AN微孔板和木质纤维素酶测定被证明是表征微生物群落代谢的快速工具，如TS负荷之间不同的碳源利用模式所示。粪便指示菌群的卫生化在一些菌群（大肠杆菌）中实现，但并非所有菌群（梭菌属）。结果表明，比色生化测定和基于培养的技术可以快速评估共消化过程中的微生物群落动态，并且木质纤维素生物质形式的TS会影响微生物代谢活动。
