猪粪与玉米秸秆的长期中温厌氧共消化及微生物群落分析
Long-Term Mesophilic Anaerobic Co-Digestion of Swine Manure with Corn Stover and Microbial Community Analysis.
作者信息
Wang Haipeng, Lim Teng Teeh, Duong Cuong, Zhang Wei, Xu Congfeng, Yan Lei, Mei Zili, Wang Weidong
机构信息
Heilongjiang Provincial Key Laboratory of Environmental Microbiology and Recycling of Agro-Waste in Cold Region, College of Life Science and Biotechnology, Heilongjiang Bayi Agricultural University, Daqing 163319, China.
Agriculture Systems Management, Division of Food Systems and Bioengineering, University of Missouri, Columbia, MO 65211-5200, USA.
出版信息
Microorganisms. 2020 Jan 29;8(2):188. doi: 10.3390/microorganisms8020188.
Long-term anaerobic co-digestion of swine manure (SM) and corn stover (CS) was conducted using semi-continuously loaded digesters under mesophilic conditions. A preliminary test was first conducted to test the effects of loading rates, and results indicated the 3 g-VS L d was the optimal loading rate. Based on the preliminary results, a verification replicated test was conducted with 3 g-VS L d loading rate and different SM/CS ratios (1:1, 2:1 and 1:2). Results showed that a SM/CS ratio of 2/1 was optimal, based on maximum observed methane-VS generation and carbon conversion efficiency (72.56 ± 3.40 mL g and 40.59%, respectively). Amplicon sequencing analysis suggested that microbial diversity was increased with CS loading. Amino-acid-degrading bacteria were abundant in the treatment groups. Archaea could enhance biogas and methane productions.
在中温条件下，使用半连续进料消化器对猪粪（SM）和玉米秸秆（CS）进行长期厌氧共消化。首先进行了一项初步试验，以测试进料速率的影响，结果表明3 g-VS/L·d是最佳进料速率。基于初步结果，以3 g-VS/L·d的进料速率和不同的SM/CS比例（1:1、2:1和1:2）进行了验证重复试验。结果表明，基于观察到的最大甲烷-VS产量和碳转化效率（分别为72.56±3.40 mL/g和40.59%），SM/CS比例为2/1是最佳的。扩增子测序分析表明，微生物多样性随着CS负载量的增加而增加。氨基酸降解细菌在处理组中丰富。古菌可以提高沼气和甲烷产量。
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