College of Geography and Environment, Shandong Normal University, 88# East Wenhua Road, Jinan, 250014, Shandong, China.
Bull Environ Contam Toxicol. 2020 Apr;104(4):477-483. doi: 10.1007/s00128-020-02823-y. Epub 2020 Mar 19.
The application of nano-level passivating agents in the remediation of soil heavy metal pollution has received widespread attention, but its harm to soil animals should also be addressed. This study explored the effect of three nanomaterials-nanohydroxyapatite apatite (n-HAP), nano zeolite (n-zeolite), and nanometer iron oxide (n-FeO), on catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) activity and malondialdehyde (MDA) content through filter paper contact test. The effects of nanomaterials spiked at 1.5%wt of soils on earthworm avoidance behavior were also be studied, and the crystallinity and surface charge of three nanomaterials were characterized. The results showed that the activities of CAT, SOD and POD and the content of MDA have been changed at different level. And earthworms have obvious avoidance behavior to the three kinds of nanomaterials. Therefore, nanomaterials do have adverse effects on earthworms, and their biological toxicity should be considered when selecting passivating agents for soil heavy metal pollution remediation.
纳米级钝化剂在土壤重金属污染修复中的应用受到广泛关注，但也应关注其对土壤动物的危害。本研究通过滤纸接触试验，探讨了三种纳米材料-纳米羟基磷灰石（n-HAP）、纳米沸石（n-zeolite）和纳米氧化铁（n-FeO）对过氧化氢酶（CAT）、过氧化物酶（POD）和超氧化物歧化酶（SOD）活性以及丙二醛（MDA）含量的影响。还研究了土壤中添加 1.5%wt 的纳米材料对蚯蚓回避行为的影响，并对三种纳米材料的结晶度和表面电荷进行了表征。结果表明，CAT、SOD 和 POD 的活性以及 MDA 的含量均发生了不同程度的变化。而且蚯蚓对三种纳米材料表现出明显的回避行为。因此，纳米材料确实对蚯蚓有不良影响，在选择土壤重金属污染修复用钝化剂时应考虑其生物毒性。
