Wu J T
Department of Obstetrics and Gynecology, University of Massachusetts Medical School, Worcester 01655.
Biol Reprod. 1988 Dec;39(5):1021-6. doi: 10.1095/biolreprod39.5.1021.
The rate of estrone (E1)----estradiol-17 beta (E2) or E2----E1 conversion catalyzed by 17 beta-hydroxysteroid dehydrogenase (17 beta-HSD) activity was determined for each mouse embryo in modified F-10 medium containing 0.95 microM 3H-E1 or 3H-E2. During delayed implantation, the E1----E2 conversion rate was decreased (p less than 0.005) from 5.69 +/- 0.34 fmol/h/blastocyst on Day 5 to 3.50 +/- 0.46 fmol/h/blastocyst on Day 9, whereas E2----E1 was increased (p less than 0.005) from 7.44 +/- 1.08 to 18.60 +/- 2.04 fmol/h/blastocyst. After estrogen injection, the Day 9 implanting blastocyst showed an increase (p less than 0.005) in E1----E2 conversion to 9.05 +/- 0.64 fmol/h/blastocyst but a slight, insignificant decrease in E2----E1 conversion to 14.2 +/- 1.82 fmol/h/blastocyst. A similar trend was also observed in Day 5 implanting blastocysts when compared to Day 5 delayed blastocysts. Thus, 17 beta-HSD activity in delayed blastocysts favors E2----E1 over E1----E2 conversion in a ratio of 5:1. After estrogen induction of implantation, the E1----E2 conversion rate is stimulated and the ratio of E2----E1 to E1----E2 rate is decreased to 1.5:1. The results suggest that 17 beta-HSD activity may be involved in blastocyst implantation.
在含有0.95微摩尔3H-雌酮（E1）或3H-雌二醇-17β（E2）的改良F-10培养基中，测定了每只小鼠胚胎由17β-羟类固醇脱氢酶（17β-HSD）活性催化的雌酮（E1）向雌二醇-17β（E2）或E2向E1转化的速率。在延迟着床期间，E1向E2的转化率从第5天的5.69±0.34飞摩尔/小时/囊胚降至第9天的3.50±0.46飞摩尔/小时/囊胚（p<0.005），而E2向E1的转化率从7.44±1.08增至18.60±2.04飞摩尔/小时/囊胚（p<0.005）。注射雌激素后，第9天着床的囊胚E1向E2的转化率增加（p<0.005）至9.05±0.64飞摩尔/小时/囊胚，而E2向E1的转化率略有下降但无统计学意义，降至14.2±1.82飞摩尔/小时/囊胚。与第5天延迟的囊胚相比，第5天着床的囊胚也观察到类似趋势。因此，延迟囊胚中的17β-HSD活性有利于E2向E1的转化，其比例为5:1，超过E1向E2的转化。雌激素诱导着床后，E1向E2的转化率受到刺激，E2向E1与E1向E2转化率的比例降至1.5:1。结果表明，17β-HSD活性可能与囊胚着床有关。
