植入前大鼠和小鼠囊胚中17β-羟基类固醇脱氢酶活性的存在。
The presence of 17 beta-hydroxysteroid dehydrogenase activity in preimplantation rat and mouse blastocysts.
作者信息
Wu J T, Lin G M
出版信息
J Exp Zool. 1982 Mar 1;220(1):121-4. doi: 10.1002/jez.1402200116.
When Day 5 rat blastocysts and Day 4 and 5 mouse blastocysts were cultured in 53 microliters of medium containing 1340 or 2680 pg [3H]estradiol (E2), large amounts of [3H]estrone (E1) were detected in the medium at daily intervals for up to 5 days. This indicates the presence of 17 beta-hydroxysteroid dehydrogenase in the embryos. The activity was higher at a higher concentration of E2 and was also higher in mouse than in rat blastocysts. In the mouse, the activity was higher in Day 5 than Day 4 blastocysts during the first day in culture; then it decreased in Day 5 but increased in Day 4 blastocysts. The importance of E2 in embryonic development and implantation as suggested by others may be related to the activity of 17 beta-hydroxysteroid dehydrogenase.
将第5天的大鼠囊胚以及第4天和第5天的小鼠囊胚培养于含有1340或2680皮克[³H]雌二醇（E2）的53微升培养基中，在长达5天的时间里，每天都能在培养基中检测到大量的[³H]雌酮（E1）。这表明胚胎中存在17β-羟基类固醇脱氢酶。在较高浓度的E2条件下，该活性更高，并且在小鼠囊胚中的活性也高于大鼠囊胚。在小鼠中，培养第一天时，第5天囊胚的活性高于第4天囊胚；然后第5天囊胚的活性下降，而第4天囊胚的活性增加。其他人提出的E2在胚胎发育和着床中的重要性可能与17β-羟基类固醇脱氢酶的活性有关。
Zotero 插件