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长链非编码RNA MACC1-AS1是宫颈鳞状细胞癌中微小RNA-34a的潜在海绵分子，并上调细胞周期蛋白依赖性激酶6。

Long noncoding RNA MACC1-AS1 is a potential sponge of microRNA-34a in cervical squamous cell carcinoma and upregulates cyclin-dependent kinase 6.

作者信息

Jin Jieqiong, Chen Xuemei, Chen Jing, Geng Xin

机构信息

Department of Gynaecology, Qingdao Jiaozhou Central Hospital, Qingdao, Shandong 266300, P.R. China.

Department of Obstetrics and Gynecology, Wuhan Third Hospital (Tongren Hospital of Wuhan University), Wuhan, Hubei 430060, P.R. China.

出版信息

Oncol Lett. 2020 Mar;19(3):2339-2345. doi: 10.3892/ol.2020.11346. Epub 2020 Jan 24.

DOI:10.3892/ol.2020.11346
PMID:32194733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7039179/
Abstract

The present study aimed to investigate the role of long noncoding RNA MACC1-AS1 in cervical squamous cell carcinoma (CSCC). In the present study MACC1-AS1 expression as analyzed using reverse transcription-quantitative PCR. The interactions between MACC1-AS1 and miR-34a was analyzed via overexpression experiments. Cell cycle and proliferation analyses were performed to analyze the roles of MACC1-AS1 in regulating cancer cell cycle progression and cell proliferation. It was observed that MACC1-AS1 was upregulated in CSCC, and its expression levels were elevated with the increase in clinical stage. Bioinformatics analysis revealed that MACC1-AS1 may be a sponge of miR-34a, which can target cyclin-dependent kinase 6 (CDK6). In CSCC cells, MACC1-AS1 overexpression led to upregulation of CDK6, while miR-34a overexpression had the opposite effect and reduced the effects of MACC1-AS1 overexpression in co-transfected cells. Cell cycle and proliferation analyses demonstrated that MACC1-AS1 and CDK6 promoted cell cycle progression and cell proliferation. By contrast, miR-34a had the opposite effect on cell cycle proliferation and cell proliferation, reducing the effects induced by MACC1-AS1 overexpression. Therefore, the lncRNA MACC1-AS1 may serve as a sponge of miR-34a to upregulate CDK6, thereby promoting cell cycle progression and cell proliferation.

摘要

本研究旨在探讨长链非编码RNA MACC1-AS1在宫颈鳞状细胞癌（CSCC）中的作用。在本研究中，使用逆转录定量PCR分析MACC1-AS1的表达。通过过表达实验分析MACC1-AS1与miR-34a之间的相互作用。进行细胞周期和增殖分析以分析MACC1-AS1在调节癌细胞周期进程和细胞增殖中的作用。结果观察到，MACC1-AS1在CSCC中上调，并且其表达水平随着临床分期的增加而升高。生物信息学分析显示，MACC1-AS1可能是miR-34a的海绵，miR-34a可以靶向细胞周期蛋白依赖性激酶6（CDK6）。在CSCC细胞中，MACC1-AS1过表达导致CDK6上调，而miR-34a过表达则产生相反的效果，并降低了共转染细胞中MACC1-AS1过表达的作用。细胞周期和增殖分析表明，MACC1-AS1和CDK6促进细胞周期进程和细胞增殖。相比之下，miR-34a对细胞周期增殖和细胞增殖具有相反的作用，降低了MACC1-AS1过表达诱导的作用。因此，lncRNA MACC1-AS1可能作为miR-34a的海绵上调CDK6，从而促进细胞周期进程和细胞增殖。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/da77b12bac28/ol-19-03-2339-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/2a35b611f454/ol-19-03-2339-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/c9fe9a0d3acc/ol-19-03-2339-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/881c0f4b6314/ol-19-03-2339-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/da77b12bac28/ol-19-03-2339-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/2a35b611f454/ol-19-03-2339-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/c9fe9a0d3acc/ol-19-03-2339-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/881c0f4b6314/ol-19-03-2339-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4ecf/7039179/da77b12bac28/ol-19-03-2339-g03.jpg

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