Man Shuhong, Li Xiaohan, Zhu Wei
Department of Gynaecology, Weifang People's Hospital, Weifang, Shandong 261000, P.R. China.
School of Public Health, Shandong University, Jinan, Shandong 250012, P.R. China.
Oncol Lett. 2021 Jul;22(1):502. doi: 10.3892/ol.2021.12763. Epub 2021 Apr 29.
Although long non-coding RNA (lncRNA) PSMG3-AS1 has been reported to participate in cancer biology, its role in cervical squamous cell carcinoma (CSCC) is unknown. The present study aimed to investigate the role of the lncRNA PSMG3-AS1 in CSCC. The expression levels of PSMG3-AS1 in both CSCC and non-tumor tissues from 64 patients with CSCC were measured by reverse transcription-quantitative PCR. The potential interaction between miR-4417 and PSMG3-AS1 was predicted using IntaRNA 2.0. Overexpression of miR-4417 and PSMG3-AS1 were achieved in CSCC cells to further explore the potential interaction between them. The effects of overexpression of miR-4417 and PSMG3-AS1 on CSCC cell invasion and migration were assessed by Transwell assay. The results revealed that PSMG3-AS1 expression was upregulated in CSCC tissues, and its high expression levels predicted a poor survival in patients with CSCC. miR-4417 expression was downregulated in CSCC tissues and was inversely correlated with PSMG3-AS1 expression. Moreover, miR-4417 was predicted to interact with PSMG3-AS1. In CSCC cells, overexpression of miR-4417 decreased the expression levels of PSMG3-AS1, while overexpression of PSMG3-AS1 did not affect miR-4417 expression. Transwell assay demonstrated that overexpression of PSMG3-AS1 increased CSCC cell invasion and migration. However, overexpression of miR-4417 inhibited CSCC cell invasion and migration, and attenuated the effects of PSMG3-AS1 overexpression in CSCC cells. In conclusion, the present study indicated that miR-4417 may target PSMG3-AS1 to suppress cancer cell invasion and migration in CSCC.
尽管已有报道称长链非编码RNA（lncRNA）PSMG3-AS1参与癌症生物学过程，但其在宫颈鳞状细胞癌（CSCC）中的作用尚不清楚。本研究旨在探讨lncRNA PSMG3-AS1在CSCC中的作用。采用逆转录定量PCR检测64例CSCC患者的CSCC组织和非肿瘤组织中PSMG3-AS1的表达水平。使用IntaRNA 2.0预测miR-4417与PSMG3-AS1之间的潜在相互作用。在CSCC细胞中实现miR-4417和PSMG3-AS1的过表达，以进一步探索它们之间的潜在相互作用。通过Transwell实验评估miR-4417和PSMG3-AS1过表达对CSCC细胞侵袭和迁移的影响。结果显示，PSMG3-AS1在CSCC组织中表达上调，其高表达水平预示着CSCC患者的不良生存。miR-4417在CSCC组织中表达下调，且与PSMG3-AS1表达呈负相关。此外，预测miR-4417与PSMG3-AS1相互作用。在CSCC细胞中，miR-4417过表达降低了PSMG3-AS1的表达水平，而PSMG3-AS1过表达不影响miR-4417的表达。Transwell实验表明，PSMG3-AS1过表达增加了CSCC细胞的侵袭和迁移。然而，miR-4417过表达抑制了CSCC细胞的侵袭和迁移，并减弱了PSMG3-AS1过表达对CSCC细胞的影响。总之，本研究表明miR-4417可能靶向PSMG3-AS1以抑制CSCC中的癌细胞侵袭和迁移。
