Fan Ruicheng, Wang Heng, Zhang Liyuan, Ma Teng, Tian Yanping, Li Hongli
Department of Histology and Embryology, College of Basic Medicine, Army Medical University, Chongqing, China.
Battalion 3, College of Basic Medicine, Army Medical University, Chongqing, China.
J Cancer. 2020 Jan 29;11(7):1949-1958. doi: 10.7150/jca.38847. eCollection 2020.
Glioma associates with high malignancy and poor prognosis for traditional treatment. Oleanolic acid (OA) has been confirmed to have an inhibitory effect on different kinds of tumors, while accompanying with low efficiency because of its large molecular mass and low solubility. Nanoliposome is an appropriate drug delivery system that can compensate for the limitations of traditional insoluble drugs, involving improvement of their solubility, stability and lipophilicity. In the present study, we comprised of OA covered with nanoliposomes, named OA, to observe antitumor effects on U87 glioma cells. The results showed that OA raised the solubility and oil-water partition coefficient. OA suppressed proliferation of U87 glioma cells, and also had an anticancer effect on U87 glioma cells, which was found to be higher than that of OA. Moreover, treatment with OA induced apoptosis and degraded migration ability by caspase-3 pathway. In conclusion, our results demonstrated that OA covered with nanoliposomes led to enhanced anticancer effects by suppressing proliferation, migration and invasion abilities. The findings may provide a reliable reference for development of new anti-cancer drugs.
胶质瘤具有高恶性程度，传统治疗预后较差。齐墩果酸（OA）已被证实对多种肿瘤具有抑制作用，但由于其分子质量大、溶解度低，效果欠佳。纳米脂质体是一种合适的药物递送系统，可弥补传统不溶性药物的局限性，包括提高其溶解度、稳定性和亲脂性。在本研究中，我们制备了包裹有纳米脂质体的OA，命名为OA，以观察其对U87胶质瘤细胞的抗肿瘤作用。结果表明，OA提高了溶解度和油水分配系数。OA抑制U87胶质瘤细胞的增殖，对U87胶质瘤细胞也有抗癌作用，且发现其抗癌效果高于OA。此外，OA处理通过半胱天冬酶-3途径诱导细胞凋亡并降低迁移能力。总之，我们的结果表明，包裹有纳米脂质体的OA通过抑制增殖、迁移和侵袭能力而增强了抗癌效果。这些发现可能为新型抗癌药物的开发提供可靠参考。
