Hoyle H W, Smith L A, Williams R J, Przyborski S A
Department of Biosciences, Durham University, South Road, Durham DH1 3LE, UK.
Department of Engineering, Durham University, South Road, Durham DH1 3LE, UK.
Interface Focus. 2020 Apr 6;10(2):20190090. doi: 10.1098/rsfs.2019.0090. Epub 2020 Feb 14.
As the field of tissue engineering continues to advance rapidly, so too does the complexity of cell culture techniques used to generate tissue constructs, with the overall aim of mimicking the microenvironment. This complexity typically comes at a cost with regards to the size of the equipment required and associated expenses. We have developed a small, low-cost bioreactor system which overcomes some of the issues of typical bioreactor systems while retaining a suitable scale for the formation of complex tissues. Herein, we have tested this system with three cell populations/tissues: the culture of hepatocellular carcinoma cells, where an improved structure and basic metabolic function is seen; the culture of human pluripotent stem cells, in which the cultures can form more heterogeneous tissues resembling the teratoma and liver tissue slices, in which improved maintenance of cellular viability is seen over the 3 days tested. This system has the flexibility to be used for a variety of further uses and has the potential to provide a more accessible alternative to current bioreactor technologies.
随着组织工程领域的迅速发展，用于生成组织构建体的细胞培养技术的复杂性也在增加，其总体目标是模拟微环境。这种复杂性通常在所需设备的尺寸和相关费用方面带来成本。我们开发了一种小型、低成本的生物反应器系统，该系统克服了典型生物反应器系统的一些问题，同时为复杂组织的形成保留了合适的规模。在此，我们用三种细胞群体/组织测试了该系统：肝细胞癌细胞的培养，观察到结构改善和基本代谢功能；人多能干细胞的培养，其中培养物可形成更类似于畸胎瘤的异质组织以及肝组织切片，在测试的3天内观察到细胞活力的维持得到改善。该系统具有用于多种进一步用途的灵活性，并且有可能为当前的生物反应器技术提供更易于使用的替代方案。
