Esmail Asiyah, Pereira João R, Zoio Patrícia, Silvestre Sara, Menda Ugur Deneb, Sevrin Chantal, Grandfils Christian, Fortunato Elvira, Reis Maria A M, Henriques Célia, Oliva Abel, Freitas Filomena
UCIBIO-REQUIMTE, Chemistry Department, Nova School of Sciences and Technology, 2829-516 Caparica, Portugal.
ITQB NOVA-Instituto de Tecnologia Química e Biológica António Xavier, Nova University Lisbon, 2780-157 Oeiras, Portugal.
Polymers (Basel). 2021 Mar 27;13(7):1056. doi: 10.3390/polym13071056.
Poly(hydroxyalkanoates) (PHAs) with differing material properties, namely, the homopolymer poly(3-hydroxybutyrate), P(3HB), the copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), with a 3HV content of 25 wt.% and a medium chain length PHA, and mcl-PHA, mainly composed of 3-hydroxydecanoate, were studied as scaffolding material for cell culture. P(3HB) and P(3HB-co-3HV) were individually spun into fibers, as well as blends of the mcl-PHA with each of the scl-PHAs. An overall biopolymer concentration of 4 wt.% was used to prepare the electrospinning solutions, using chloroform as the solvent. A stable electrospinning process and good quality fibers were obtained for a solution flow rate of 0.5 mL h, a needle tip collector distance of 20 cm and a voltage of 12 kV for P(3HB) and P(3HB-co-3HV) solutions, while for the mcl-PHA the distance was increased to 25 cm and the voltage to 15 kV. The scaffolds' hydrophilicity was significantly increased under exposure to oxygen plasma as a surface treatment. Complete wetting was obtained for the oxygen plasma treated scaffolds and the water uptake degree increased in all treated scaffolds. The biopolymers crystallinity was not affected by the electrospinning process, while their treatment with oxygen plasma decreased their crystalline fraction. Human dermal fibroblasts were able to adhere and proliferate within the electrospun PHA-based scaffolds. The P(3HB-co-3HV): mcl-PHA oxygen plasma treated scaffold highlighted the most promising results with a cell adhesion rate of 40 ± 8%, compared to 14 ± 4% for the commercial oxygen plasma treated polystyrene scaffold Alvetex. Scaffolds based on P(3HB-co-3HV): mcl-PHA blends produced by electrospinning and submitted to oxygen plasma exposure are therefore promising biomaterials for the development of scaffolds for tissue engineering.
研究了具有不同材料特性的聚羟基脂肪酸酯（PHA），即均聚物聚（3-羟基丁酸酯）P（3HB）、3HV含量为25 wt.%的共聚物聚（3-羟基丁酸酯-co-3-羟基戊酸酯）P（3HB-co-3HV）以及主要由3-羟基癸酸酯组成的中链长度PHA（mcl-PHA）作为细胞培养的支架材料。P（3HB）和P（3HB-co-3HV）分别纺成纤维，mcl-PHA也分别与每种短链长度PHA（scl-PHA）制成共混物。使用氯仿作为溶剂，以4 wt.%的总生物聚合物浓度制备静电纺丝溶液。对于P（3HB）和P（3HB-co-3HV）溶液，当溶液流速为0.5 mL/h、针尖与收集器距离为20 cm且电压为12 kV时，可获得稳定的静电纺丝过程和高质量的纤维；而对于mcl-PHA，距离增加到25 cm，电压增加到15 kV。作为表面处理，在氧气等离子体处理下，支架的亲水性显著增加。氧气等离子体处理的支架实现了完全润湿，且所有处理过的支架的吸水率都有所增加。静电纺丝过程不影响生物聚合物的结晶度，而氧气等离子体处理会降低其结晶分数。人皮肤成纤维细胞能够在基于PHA的静电纺丝支架内黏附并增殖。经氧气等离子体处理的P（3HB-co-3HV）：mcl-PHA支架表现出最有前景的结果，细胞黏附率为40±8%，相比之下，商业氧气等离子体处理的聚苯乙烯支架Alvetex的细胞黏附率为14±4%。因此，通过静电纺丝制备并经氧气等离子体处理的基于P（3HB-co-3HV）：mcl-PHA共混物的支架是用于组织工程支架开发的有前景的生物材料。
