Mirakhorli Neda, Norolah Zahra, Foruzandeh Samira, Shafizade Fateme, Nikookhah Farzaneh, Saffar Behnaz, Ansari Omid
Department of Plant Breeding and Biotechnology, Shahrekord University, Shahrekord, Iran.
Department of Fisheries and Environmental Science, Shahrekord University, Shahrekord, Iran.
Iran J Biotechnol. 2019 Sep 1;17(3):e1562. doi: 10.29252/ijb.1562. eCollection 2019 Sep.
Defensin peptide isolated from plants are often heterogeneous in length, sequence and structure, but they are mostly small, cationic and amphipathic. Plant defensins exhibit broad-spectrum antibacterial and antifungal activities against Gram-positive and Gram-negative bacteria, fungi and etc. Plant defensins also play an important role in innate immunity, such as heavy metal and some abiotic stresses tolerance.
In this paper, broad-spectrum activities, antimicrobial and heavy metal absorption, of a recombinant plant defensin were studied.
gene, a modified plant defensin gene, was cloned in pBISN1-IN (EU886197) plasmid, recombinant protein was produced by transient expression via Agroinfiltration method in common bean. The recombinant protein was tested for antibacterial activity against Gram-negative, Gram-positive bacteria and Fusarium sp. the effects of different treatments on heavy metal zinc absorption by this peptide were tested.
We confirmed the antibacterial activities of this peptide against Gram-negative ( and ) and Gram-positive ( and ) bacteria, and antifungal activities of this peptide against Fusarium spp. ( and ). High metal absorption coefficient for this peptide was also observed.
Out of six actinobacterial isolates, VITVAMB 1 possessed the most efficient RO-16 decolorization property. It decolorized 85.6% of RO-16 (250 mg L) within 24hrs. Isolate VITVAMB 1 was identified to be sp. Maximum dye decolorization occurred at pH 8, temperature 35oC, 3% salt concentration and a dye concentration of 50 mg L.
Results suggesting that modified defensin peptide facilitates a broader range of defense activities. dedefensins are an important part of the innate immune system in eukaryotes. These molecules have multidimensional properties that making them promising agents for therapeutic drugs.
从植物中分离出的防御素肽在长度、序列和结构上通常具有异质性，但它们大多体积小、呈阳离子性且具有两亲性。植物防御素对革兰氏阳性菌、革兰氏阴性菌、真菌等具有广谱抗菌和抗真菌活性。植物防御素在先天免疫中也发挥着重要作用，例如对重金属和一些非生物胁迫的耐受性。
本文研究了一种重组植物防御素的广谱活性、抗菌和重金属吸收能力。
将一个经过修饰的植物防御素基因克隆到pBISN1-IN（EU886197）质粒中，通过农杆菌浸润法在普通菜豆中进行瞬时表达来产生重组蛋白。测试了该重组蛋白对革兰氏阴性菌、革兰氏阳性菌和镰刀菌属的抗菌活性，以及不同处理对该肽吸收重金属锌的影响。
我们证实了该肽对革兰氏阴性菌（ 和 ）和革兰氏阳性菌（ 和 ）的抗菌活性，以及对镰刀菌属（ 和 ）的抗真菌活性。还观察到该肽具有较高的金属吸收系数。
在六种放线菌分离株中，VITVAMB 1具有最有效的RO - 16脱色特性。它在24小时内使85.6%的RO - 16（250毫克/升）脱色。分离株VITVAMB 1被鉴定为 种。最大染料脱色发生在pH 8、温度35℃、盐浓度3%和染料浓度50毫克/升的条件下。
结果表明修饰后的防御素肽促进了更广泛的防御活性。去防御素是真核生物先天免疫系统的重要组成部分。这些分子具有多维度特性，使其成为有前途的治疗药物。
需注意，原文中部分细菌名称等未完整给出具体内容，译文按原样保留了相关表述。
