Natarajan Purushothaman, Ahn Ezekiel, Reddy Umesh K, Perumal Ramasamy, Prom Louis K, Magill Clint
Department of Biology, Gus R. Douglass Institute, West Virginia State University, West Virginia, WV, United States.
Department of Plant Pathology and Microbiology, Texas A & M University, College Station, TX, United States.
Front Genet. 2021 Aug 11;12:722519. doi: 10.3389/fgene.2021.722519. eCollection 2021.
Gene expression was analyzed at 0- and 24-h post-inoculation of two inbred sorghum cultivars known to differ in response to inoculation with , the fungal pathogen that causes anthracnose. QL3 is reported to have quantitative resistance, while Theis is susceptible to most pathotypes of the pathogen; RNASeq identified over 3,000 specific genes in both cultivars as showing significant changes in expression following inoculation; in all but one gene, the changes in QL3 and Thies were in the same direction. Many other genes showed significant changes in only one of the two cultivars. Overall, more genes were downregulated than upregulated. Differences in changes in expression levels of a few genes suggested potential roles for the difference in disease response between QL3 and Theis, but did not identify known resistance genes. Gene ontology (GO) and pathway enrichment analysis identified upregulation of 23 transcription factor encoding genes as well as genes involved in the production of secondary metabolites, which are part of a typical host defense reaction.
在接种已知对引起炭疽病的真菌病原体接种反应不同的两个自交系高粱品种后0小时和24小时分析基因表达。据报道，QL3具有定量抗性，而Theis对该病原体的大多数致病型敏感；RNA测序在两个品种中均鉴定出3000多个特定基因，这些基因在接种后表达有显著变化；除一个基因外，QL3和Theis的变化方向相同。许多其他基因仅在两个品种中的一个中显示出显著变化。总体而言，下调的基因比上调的基因更多。少数基因表达水平变化的差异表明QL3和Theis之间疾病反应差异的潜在作用，但未鉴定出已知的抗性基因。基因本体（GO）和通路富集分析确定了23个编码转录因子的基因以及参与次生代谢产物产生的基因上调，这些基因是典型宿主防御反应的一部分。
