Gorza L, Schiaffino S, Vitadello M
Institute of General Pathology, University of Padova, Italy.
Brain Res. 1988 Aug 9;457(2):360-6. doi: 10.1016/0006-8993(88)90707-x.
Using the anti-neurofilament monoclonal antibody iC8 we report here that muscle fibers of the conduction system of the adult and developing rabbit heart express a cytoskeletal protein antigenically and electrophoretically similar to the middle subunit of neurofilaments (NF-M). In the 11-day embryo a number of cardiac muscle cells also express a neural crest surface marker recognized by the monoclonal antibody HNK-1. Both markers are found in many cells of the 3rd and 4th branchial arches, which are populated by cells of neural crest origin. In the 11-day embryo cells of the 4th branchial arch are in close proximity to and intermingled with the atrial myocardium: cells co-expressing sarcomeric myosin heavy chain with iC8 and HNK-1 immunoreactivity are seen at these sites. The findings suggest that conduction tissue cells of the rabbit heart originate from a population of neural crest-derived cells migrating from the branchial arches into the developing heart.
我们在此报告，使用抗神经丝单克隆抗体iC8发现，成年和发育中的兔心脏传导系统的肌纤维表达一种细胞骨架蛋白，其抗原性和电泳特性与神经丝中间亚基（NF-M）相似。在11天大的胚胎中，许多心肌细胞还表达一种被单克隆抗体HNK-1识别的神经嵴表面标志物。这两种标志物都存在于第3和第4鳃弓的许多细胞中，这些细胞由神经嵴来源的细胞组成。在11天大的胚胎中，第4鳃弓的细胞与心房心肌紧密相邻并相互交织：在这些部位可见共表达肌节肌球蛋白重链以及具有iC8和HNK-1免疫反应性的细胞。这些发现表明，兔心脏的传导组织细胞起源于一群从鳃弓迁移到发育中心脏的神经嵴衍生细胞。
