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分光光度法中的误差及避免这些误差的校准程序。

Errors in Spectrophotometry and Calibration Procedures to Avoid Them.

作者信息

Reule A G

机构信息

Carl Zeiss, 7082 Oberkochen, Germany.

出版信息

J Res Natl Bur Stand A Phys Chem. 1976 Jul-Aug;80A(4):609-624. doi: 10.6028/jres.080A.060. Epub 1976 Aug 1.

DOI:10.6028/jres.080A.060
PMID:32196282
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5293527/
Abstract

Based on simple principles, spectrophotometry nevertheless demands a lot of precautions to avoid errors. The following properties of spectrophotometers will be discussed together with methods to test them: Spectral properties-wavelength accuracy, bandwidth, stray light; photometric linearity; interactions between sample and instrument-multiple reflections, polarization, divergence, sample wedge, sample tilt, optical path length (refractive index), interferences. Calibration of master instruments is feasible only by complicated procedures. With such a master instrument standards may be calibrated which greatly simplify performance checks of instruments used for practical work. For testing high quality spectrophotometers the use of emission lines and nearly neutral absorbing solid filters as standards seems to be superior, for some kinds of routine instruments the use of absorption bands and liquid filters may be necessary.

摘要

尽管分光光度法基于简单的原理，但仍需要采取许多预防措施以避免误差。将讨论分光光度计的以下特性以及测试这些特性的方法：光谱特性——波长准确度、带宽、杂散光；光度线性；样品与仪器之间的相互作用——多次反射、偏振、发散、样品楔、样品倾斜、光程长度（折射率）、干扰。主仪器的校准仅通过复杂的程序才可行。有了这样的主仪器，就可以校准标准，这大大简化了用于实际工作的仪器的性能检查。对于测试高质量的分光光度计，使用发射线和近乎中性吸收的固体滤光片作为标准似乎更具优势，对于某些常规仪器，可能需要使用吸收带和液体滤光片。

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本文引用的文献

1
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2
Efficient averaging spheres for visible and ultraviolet wavelengths.
Appl Opt. 1975 Aug 1;14(8):1940-7. doi: 10.1364/AO.14.001940.
3
Technique for measuring photometric accuracy.
Appl Opt. 1971 Jun 1;10(6):1246-53. doi: 10.1364/AO.10.001246.
4
壳聚糖/透明质酸膜稳定的载瑞德西韦纳米脂质体在冠状病毒感染治疗中的潜在应用
Neurol Int. 2023 Oct 30;15(4):1320-1338. doi: 10.3390/neurolint15040083.
4
Assessing variations in manual pipetting: An under-investigated requirement of good laboratory practice.评估手动移液的差异：良好实验室规范中一项未得到充分研究的要求。
J Mass Spectrom Adv Clin Lab. 2023 Oct 8;30:25-29. doi: 10.1016/j.jmsacl.2023.09.001. eCollection 2023 Nov.
5
A Novel Approach to Monitor the Concentration of Phosphate Buffers in the Range of 1 M to 0.1 M Using a Silicon-Based Impedance Sensor.一种使用基于硅的阻抗传感器监测 1 M 至 0.1 M 范围内磷酸盐缓冲液浓度的新方法。
Biosensors (Basel). 2023 Aug 24;13(9):841. doi: 10.3390/bios13090841.
Testing spectrophotometer linearity.
Appl Opt. 1968 Jun 1;7(6):1023-8. doi: 10.1364/AO.7.001023.
5
CAP survey to assess the extent of stray light problems in precision spectrophotometry.
Am J Clin Pathol. 1975 Jun;63(6 SUPPL):953-9.