LINC00675 通过 Wnt/β-catenin 通路抑制透明细胞肾细胞癌的增殖、迁移和侵袭。
LINC00675 suppresses proliferative, migration and invasion of clear cell renal cell carcinoma via the Wnt/β-catenin pathway.
机构信息
Department of Urology, Xingtai People's Hospital, Xingtai, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Mar;24(5):2313-2320. doi: 10.26355/eurrev_202003_20497.
OBJECTIVE
To clarify the role of LINC00675 in affecting the progression of clear cell renal cell carcinoma (ccRCC) and its potential mechanism, thus providing effective hallmarks and therapeutic targets for the clinical treatment of ccRCC.
MATERIALS AND METHODS
Differentially expressed long non-coding RNAs (lncRNAs) in renal epithelial tissues and ccRCC tissues were searched by analyzing the dataset downloaded from The Cancer Genome Atlas (TCGA) and LINC00675 was selected. LINC00675 level in ccRCC cell lines was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Overexpression model of LINC00675 model in 786-O and 769-P cells was constructed by the transfection of pcDNA3.1(+)-LINC00675 (LV-LINC00675). Changes in proliferative, migratory, and invasive capacities of 786-O and 769-P cells overexpressing LINC00675 were assessed. At last, relative levels of β-catenin, Vimentin, and N-cadherin in ccRCC cells overexpressing LINC00675 were detected by qRT-PCR and Western blot.
RESULTS
LINC00675 was downregulated in ccRCC tissues and cell lines. Overexpression of LINC00675 attenuated proliferative, migratory, and invasive capacities of 786-O and 769-P cells. Downregulation in β-catenin after overexpression of LINC00675, while Vimentin and N-cadherin levels did not change.
CONCLUSIONS
LINC00675 is downregulated in ccRCC. Overexpression of LINC00675 attenuates ccRCC to proliferate, migrate, and invade by activating the Wnt/β-catenin pathway.
目的
阐明 LINC00675 在影响透明细胞肾细胞癌（ccRCC）进展中的作用及其潜在机制，从而为 ccRCC 的临床治疗提供有效的标志物和治疗靶点。
材料与方法
通过分析从癌症基因组图谱（TCGA）下载的数据集，搜索肾上皮组织和 ccRCC 组织中差异表达的长非编码 RNA（lncRNA），并选择 LINC00675。通过定量实时聚合酶链反应（qRT-PCR）确定 ccRCC 细胞系中的 LINC00675 水平。通过 pcDNA3.1（+）-LINC00675（LV-LINC00675）转染构建 786-O 和 769-P 细胞中 LINC00675 的过表达模型。检测过表达 LINC00675 后 786-O 和 769-P 细胞增殖、迁移和侵袭能力的变化。最后，通过 qRT-PCR 和 Western blot 检测过表达 LINC00675 的 ccRCC 细胞中β-catenin、波形蛋白和 N-钙粘蛋白的相对水平。
结果
LINC00675 在 ccRCC 组织和细胞系中下调。过表达 LINC00675 减弱了 786-O 和 769-P 细胞的增殖、迁移和侵袭能力。过表达 LINC00675 后β-catenin 下调，而波形蛋白和 N-钙粘蛋白水平没有变化。
结论
LINC00675 在 ccRCC 中下调。过表达 LINC00675 通过激活 Wnt/β-catenin 通路减弱 ccRCC 的增殖、迁移和侵袭能力。
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