长链非编码 RNA ENST00000434223 通过 Wnt/ hygro-catenin 信号通路抑制肾癌的进展。
Long non-coding RNA ENST00000434223 inhibits the progression of renal cancer through Wnt/hygro-catenin signaling pathway.
机构信息
Department of Urology, Gansu Provincial Hospital, Lanzhou, China.
出版信息
Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):6868-6877. doi: 10.26355/eurrev_201908_18726.
OBJECTIVE
The purpose of this study was to determine the function of long non-coding RNA (LncRNA) ENST00000434223 (Lnc ENST) in renal carcinoma, and to explore the potential molecular mechanism.
PATIENTS AND METHODS
Quantitative Real-Time-Polymerase Chain Reaction (qRT-PCR) was used to detect the expressions of lncRNA ENST00000434223 and Wnt/β-catenin pathway-related mRNAs in tissues and cells of renal cancer. Chi-square test was performed to figure out the relationship between lncRNA ENST00000434223 and clinic-pathologic features of renal cancer patients. Besides, si-NC, si-ENST00000434223, pcDNA-NC and pcDNA-ENST00000434223 were transfected into renal cancer cells. The proliferative ability, metastasis and invasiveness of cells were detected using Cell Counting Kit-8 (CCK-8) and transwell assay, respectively. Lastly, the activation of the Wnt/hygro-catenin signal transduction pathway was evaluated by TOP/FOP Wnt Luciferase reporter assay and Western blot.
RESULTS
The expressions of Wnt2b and β-catenin were significantly increased in renal carcinoma, while E-cadherin was markedly down-regulated. Lowly expressed ENST00000434223 was involved in the poor prognosis of patients with renal cancer. In addition, down-regulating ENST00000434223 could enhance the viability, metastasis and invasiveness of renal cancer cells. However, overexpressing ENST00000434223 remarkably weakened the above cell functions. At the same time, interference or overexpression of ENST00000434223 could affect the expression level of proteins related to the Wnt/β-catenin signal pathway.
CONCLUSIONS
LncRNA ENST00000434223 inhibits the progression of renal cancer through the Wnt/shell-catenin signal pathway.
目的
本研究旨在探讨长链非编码 RNA（LncRNA）ENST00000434223（LncENST）在肾细胞癌中的作用及其潜在的分子机制。
方法
采用实时荧光定量聚合酶链反应（qRT-PCR）检测肾细胞癌组织和细胞中 LncRNA ENST00000434223 和 Wnt/β-catenin 通路相关 mRNAs 的表达。采用卡方检验分析 LncRNA ENST00000434223 与肾细胞癌患者临床病理特征的关系。此外，将 si-NC、si-ENST00000434223、pcDNA-NC 和 pcDNA-ENST00000434223 转染至肾癌细胞，采用细胞计数试剂盒-8（CCK-8）和 Transwell 实验分别检测细胞的增殖能力、迁移和侵袭能力。最后，通过 TOP/FOP Wnt 荧光素酶报告基因检测和 Western blot 评估 Wnt/β-catenin 信号转导通路的激活情况。
结果
肾癌细胞中 Wnt2b 和 β-catenin 的表达明显增加，而 E-cadherin 的表达明显下调。低表达的 ENST00000434223 与肾细胞癌患者的不良预后有关。此外，下调 ENST00000434223 可增强肾癌细胞的活力、迁移和侵袭能力。然而，过表达 ENST00000434223 可显著减弱上述细胞功能。同时，ENST00000434223 的干扰或过表达可影响 Wnt/β-catenin 信号通路相关蛋白的表达水平。
结论
LncRNA ENST00000434223 通过 Wnt/β-catenin 信号通路抑制肾癌细胞的进展。
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