The State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
Department of Geriatric Dentistry, West China Hospital of Stomatology, Sichuan University, Chengdu, China.
J Dent Res. 2020 Jul;99(7):847-854. doi: 10.1177/0022034520912181. Epub 2020 Mar 20.
The pyruvate oxidase (SpxB)-dependent production of HO is widely distributed among oral commensal streptococci. Several studies confirmed the ability of HO to antagonize susceptible oral bacterial species, including caries-associated as well as several periodontal pathobionts. Here we report a potential mechanism to bolster oral commensal streptococcal HO production by magnesium (Mg) supplementation. Magnesium is a cofactor for SpxB catalytic activity, and supplementation increases the production of HO in vitro. We demonstrate that Mg affects transcription and SpxB abundance in and . The competitiveness of low-passage commensal streptococcal clinical isolates is positively influenced in antagonism assays against . In growth conditions normally selective for , Mg supplementation is able to increase the abundance of in dual-species biofilms. Using an in vivo biophotonic imaging platform, we further demonstrate that dietary Mg supplementation significantly improves oral colonization in mice. In summary, our results support a role for Mg supplementation as a potential prebiotic to promote establishment of oral health-associated commensal streptococci.
依赖于丙酮酸氧化酶（SpxB）的 HO 生成广泛存在于口腔共生链球菌中。几项研究证实了 HO 能够拮抗易感口腔细菌，包括致龋相关细菌以及几种牙周病原菌。在这里，我们报告了一种通过镁（Mg）补充来增强口腔共生链球菌 HO 生成的潜在机制。镁是 SpxB 催化活性的辅助因子，补充镁可增加体外 HO 的生成。我们证明，Mg 会影响 和 中的 转录和 SpxB 丰度。在针对 的拮抗试验中，低传代口腔共生链球菌临床分离株的竞争力受到正向影响。在通常对 具有选择性的生长条件下，Mg 补充能够增加双物种生物膜中 的丰度。我们使用体内生物发光成像平台进一步证明，膳食 Mg 补充显著改善了小鼠口腔中的 定植。总之，我们的结果支持将 Mg 补充作为一种潜在的益生元，以促进与口腔健康相关的共生链球菌的定植。
