Bardill James R, Park Daewon, Marwan Ahmed I
Department of Bioengineering, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado; Division of Pediatric Surgery, Department of Surgery, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado.
Department of Bioengineering, University of Colorado Denver Anschutz Medical Campus, Aurora, Colorado.
J Surg Res. 2020 Jul;251:262-274. doi: 10.1016/j.jss.2020.01.022. Epub 2020 Mar 17.
Myelomeningocele (MMC) is an open neural tube defect of the spinal column. Our laboratory previously introduced a reverse thermal gel (RTG) as the first in situ forming patch for in utero MMC application. To overcome the challenges of anchoring the RTG in the wet amniotic environment to improve MMC coverage, we modified the RTG to mimic the underwater adhesive properties of mussels. We have separated this study into three separate hypotheses-based components: CONCLUSIONS: The DRTG demonstrates increased elasticity, cellular scaffolding properties, and improved MMC coverage in the Grhl3 mouse model. Future studies will be translated to the preclinical ovine model to evaluate this novel gel.
脊髓脊膜膨出（MMC）是脊柱的一种开放性神经管缺陷。我们实验室之前引入了一种反向热凝胶（RTG），作为用于子宫内MMC应用的首个原位形成贴片。为了克服在湿羊水环境中固定RTG以改善MMC覆盖的挑战，我们对RTG进行了改良，以模仿贻贝的水下粘附特性。我们将这项研究分为三个基于假设的独立部分：结论：在Grhl3小鼠模型中，DRTG表现出增加的弹性、细胞支架特性以及改善的MMC覆盖。未来的研究将转化到临床前绵羊模型中以评估这种新型凝胶。
