Zhao Wenlei, Wei Hongliang, Liu Fengjie, Ran Chao
Department of Radiology, Yantai Yuhuangding Hospital, The Affiliated Hospital of Qingdao University, Yantai, Shandong, 264000, PR China.
Department of Radiology, Yantai Yuhuangding Hospital, The Affiliated Hospital of Qingdao University, Yantai, Shandong, 264000, PR China.
Photodiagnosis Photodyn Ther. 2020 Jun;30:101744. doi: 10.1016/j.pdpdt.2020.101744. Epub 2020 Mar 18.
Probes with long lifetime fluorescent emission are highly desirable for time-resolved fluorescence imaging to eliminate the interference from short-lived auto-fluorescence. Aromatic-imide-based thermally activated delayed fluorescence material TADF compound with high external quantum efficiency was encapsulated with Glucose-PEG2000-DSPE amphiphilic copolymer to prepare nanoprobe micelles. Glucose-PEG2000-DSPE TADF (GPDT) Micelles had good biocompatibility, biodegradability, less toxicity, and they could be efficiently transported by over expressed GLUT1 on tumor cell membrane, then efficiently targeted lysosome of HepG2 cells. GPDT Micelles had great clinical application and research development value, and be suitable for the application and popularization in fluorescence imaging technology, especially time-resolved fluorescent imaging in living cells.
具有长寿命荧光发射的探针对于时间分辨荧光成像非常理想，可消除来自短寿命自发荧光的干扰。将具有高外量子效率的基于芳族酰亚胺的热活化延迟荧光材料TADF化合物用葡萄糖-聚乙二醇2000-二硬脂酰基磷脂酰乙醇胺两亲共聚物封装，制备纳米探针胶束。葡萄糖-聚乙二醇2000-二硬脂酰基磷脂酰乙醇胺TADF（GPDT）胶束具有良好的生物相容性、生物降解性、低毒性，并且它们可以通过肿瘤细胞膜上过度表达的葡萄糖转运蛋白1（GLUT1）有效地转运，然后有效地靶向HepG2细胞的溶酶体。GPDT胶束具有很大的临床应用和研究开发价值，适用于荧光成像技术中的应用和推广，尤其是活细胞中的时间分辨荧光成像。
