转录组研究揭示了伏马菌素 B1 通过 TNF 信号通路诱导猪肾细胞凋亡。
Transcriptome study reveals apoptosis of porcine kidney cells induced by fumonisin B1 via TNF signalling pathway.
机构信息
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
出版信息
Food Chem Toxicol. 2020 May;139:111274. doi: 10.1016/j.fct.2020.111274. Epub 2020 Mar 18.
Fumonisin B (FB) is a mycotoxin that contaminates cash crops and has toxic effects on humans and livestock. However, the toxic effect of FB is not fully understood. In this study, the apoptosis mechanism of FB on porcine kidney cells (PK-15) was elucidated by transcriptome analysis. The results showed that FB observably changed the expression of mRNA in PK-15 and induced the cells of apoptosis after being exposed to 106 μM FB in vitro. Gene Ontology and KEGG enrichment analyses showed that FB exposure increased the expressions of related mRNA in TNF signalling pathway in PK-15. To verify our bioinformatics analysis, these changes were verified by qRT-PCR and Western blot assay. Furthermore, the mRNA expressions of NF-κB and its downstream genes or proteins decreased significantly (p < 0.01) after the addition of BAY11-7082, the inhibitor of NF-κB. Therefore, for the first time, we demonstrate that FB can induce apoptosis of PK-15 cells through TNF signalling pathway, and NF-κB gene is a target of FB acting on the TNF signalling pathway.
伏马菌素 B（FB）是一种污染经济作物的真菌毒素，对人类和牲畜具有毒性作用。然而，FB 的毒性作用尚未完全阐明。在这项研究中，通过转录组分析阐明了 FB 对猪肾细胞（PK-15）的凋亡机制。结果表明，FB 在体外以 106 μM FB 处理后，明显改变了 PK-15 中 mRNA 的表达，并诱导细胞凋亡。GO 和 KEGG 富集分析表明，FB 暴露增加了 TNF 信号通路中相关 mRNA 在 PK-15 中的表达。为了验证我们的生物信息学分析，通过 qRT-PCR 和 Western blot 检测验证了这些变化。此外，加入 NF-κB 抑制剂 BAY11-7082 后，NF-κB 及其下游基因或蛋白的 mRNA 表达显著降低（p < 0.01）。因此，我们首次证明 FB 可以通过 TNF 信号通路诱导 PK-15 细胞凋亡，而 NF-κB 基因是 FB 作用于 TNF 信号通路的靶基因。
Zotero 插件