Song Ning, Ma Cunxia, Guo Yuzhu, Cui Shuangshuang, Chen Shihao, Chen Zhi, Ling Yinghui, Zhang Yunhai, Liu Hongyu
Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, China.
Jiangsu Key Laboratory of Animal Genetic Breeding and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, China.
Front Vet Sci. 2024 May 21;11:1392152. doi: 10.3389/fvets.2024.1392152. eCollection 2024.
The suppressor of cytokine signaling 3 (SOCS3) is a key signaling molecule that regulates milk synthesis in dairy livestock. However, the molecular mechanism by which regulates lipid synthesis in goat milk remains unclear. This study aimed to screen for key downstream genes associated with lipid synthesis regulated by in goat mammary epithelial cells (GMECs) using RNA sequencing (RNA-seq). Goat overexpression vector (PC-SOCS3) and negative control (PCDNA3.1) were transfected into GMECs. Total RNA from cells after overexpression was used for RNA-seq, followed by differentially expressed gene (DEG) analysis, functional enrichment analysis, and network prediction. overexpression significantly inhibited the synthesis of triacylglycerol, total cholesterol, non-esterified fatty acids, and accumulated lipid droplets. In total, 430 DEGs were identified, including 226 downregulated and 204 upregulated genes, following overexpression. Functional annotation revealed that the DEGs were mainly associated with lipid metabolism, cell proliferation, and apoptosis. We found that the lipid synthesis-related genes, , were downregulated. In addition, the proliferation-related genes , , and were upregulated, and the apoptosis-related gene was downregulated. In conclusion, six DEGs were identified as key regulators of milk lipid synthesis following overexpression in GMECs. Our results provide new candidate genes and insights into the molecular mechanisms involved in milk lipid synthesis regulated by in goats.
细胞因子信号转导抑制因子3（SOCS3）是调节奶牛产奶的关键信号分子。然而，其调节山羊奶脂质合成的分子机制尚不清楚。本研究旨在利用RNA测序（RNA-seq）筛选山羊乳腺上皮细胞（GMECs）中受SOCS3调节的与脂质合成相关的关键下游基因。将山羊SOCS3过表达载体（PC-SOCS3）和阴性对照（PCDNA3.1）转染到GMECs中。过表达后细胞的总RNA用于RNA-seq，随后进行差异表达基因（DEG）分析、功能富集分析和网络预测。SOCS3过表达显著抑制了三酰甘油、总胆固醇、非酯化脂肪酸的合成以及脂滴的积累。过表达SOCS3后，共鉴定出430个差异表达基因，包括226个下调基因和204个上调基因。功能注释显示，差异表达基因主要与脂质代谢、细胞增殖和凋亡相关。我们发现脂质合成相关基因FASN、SCD和ACACA被下调。此外，增殖相关基因CCND1(、)CCNE1和PCNA被上调，凋亡相关基因BAX被下调。总之，在GMECs中过表达SOCS3后，六个差异表达基因被鉴定为乳脂合成的关键调节因子。我们的研究结果为山羊中受SOCS3调节的乳脂合成分子机制提供了新的候选基因和见解。
