从乌克兰不同膨润土矿床分离出的棘阿米巴属原生动物的形态、生理和遗传特征。
Morphological, physiological and genetic characteristics of protozoa of genus Acanthamoeba, isolated from different deposit of bentonite in Ukraine.
作者信息
Shyrobokov Volodymyr, Poniatovskyi Vadym, Chobotar Anastasiia, Sałamatin Rusłan
机构信息
Department of Microbiology, Virology and Immunology, Bogomolets National Medical University, Peremogy av. 34, 03056 Kyiv, Ukraine
Department of General Biology and Parasitology, Medical University of Warsaw, Chałubińskiego 5, 02-004 Warsaw, Poland
出版信息
Ann Parasitol. 2020;66(1):69–75. doi: 10.17420/ap6601.239.
The representatives of genus Acanthamoeba are widespread in the environment. The presence of freeliving Acanthamoeba sp. in such mineral deposits as bentonite was shown for the first time. Identification of isolated amoeba was conducted according to morphological features of trophozoites and cysts, as well as using sequencing of gene 18S RNA (amplifier GTSA.B1). The obtained data showed that isolated amoebae belong to the genotype T4 and II morphological group (cyst size <18 μm). For its growth, ”bentonite” amoebae are intensively used bacteria of the genus Cellulosimicrobium sp. as a food substrate.
棘阿米巴属的代表在环境中广泛分布。首次发现自由生活的棘阿米巴属物种存在于膨润土等矿质沉积物中。根据滋养体和包囊的形态特征以及使用18S RNA基因测序（扩增子GTSA.B1）对分离出的变形虫进行鉴定。获得的数据表明，分离出的变形虫属于基因型T4和形态学第二组（包囊大小<18μm）。“膨润土”变形虫在生长过程中大量利用纤维微菌属的细菌作为食物底物。
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