菲律宾布希湖棘阿米巴属物种的分子鉴定。
Molecular identification of Acanthamoeba sp. in Lake Buhi, Philippines.
作者信息
Hagosojos Bernardino, Masangkay Frederick, Fernandez Jill Bennet, Lazaro Jaciel Andrea, Medroso Dante Emmanuel, Olaguera Bonnie, Pastores Cristian Mark, Santos Jenirose, Milanez Giovanni
机构信息
Department of Medical Technology, Far Eastern University, Nicanor Reyes St., Manila 1015, Philippines
出版信息
Ann Parasitol. 2020;66(1):111–114. doi: 10.17420/ap6601.245.
Acanthamoeba spp. are ubiquitous in both natural and man-made environments and have been isolated in lakes, recreational pools, tap water, and air conditioning systems. Twenty surface water (SW) samples were collected from different sampling areas of Lake Buhi. Water samples were pelleted, cultured in NNA lawned with Escherichia coli and observed microscopically. 10% of samples (2/20) were positive for amoebic growth and were furthered tested using molecular techniques. Polymerase chain reaction showed the presence of Acanthamoeba sp. DNA. The presence of potentially pathogenic Acanthamoeba sp. poses a public health concern. The formulation of policies for proper information dissemination and control measures to avert the contraction of pathogenic FLA as well as other WBPP should be seriously considered.
棘阿米巴属在自然和人造环境中均普遍存在，已在湖泊、休闲泳池、自来水和空调系统中分离出来。从布希湖的不同采样区域采集了20份地表水（SW）样本。将水样沉淀，在接种有大肠杆菌的非营养琼脂平板上培养，并进行显微镜观察。10%的样本（2/20）阿米巴生长呈阳性，并使用分子技术进一步检测。聚合酶链反应显示存在棘阿米巴属DNA。潜在致病性棘阿米巴属的存在引发了公共卫生问题。应认真考虑制定适当信息传播政策和控制措施，以避免感染致病性自由生活阿米巴以及其他水源性疾病。
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