Department of Medical Technology, Faculty of Pharmacy, University of Santo Tomas, Manila, 1008, Philippines.
School of Allied Health Sciences, National University, Manila, Philippines.
J Water Health. 2024 Sep;22(9):1677-1682. doi: 10.2166/wh.2024.156. Epub 2024 Aug 8.
Natural hot springs are ideal places and environmental matrices that offer relaxation to people and microorganisms of different types. A total of 40 surface water samples were collected from the five identified collection sites, eight water samples for each site. Collection sites are designated 200 m apart to cover the entire study site. Surface water samples were collected approximately 10-20 cm from the surface. Water samples were filtered, cultured, and microscopically observed for 14 days. After 14 days of cultivation, eight (20%) water samples revealed cystic and trophozoite stages. Polymerase chain reaction using JDP1 and JDP2 specific primers confirmed the presence of spp. from two of our isolates in the hot spring, isolates 1.1 and 5.1. Further sequencing revealed that the isolates are T20 and genotype T7. Sequences were deposited to GenBank and were assigned accession numbers PP741726 and PP741727, respectively. The isolation of spp. in hot springs has significant health implications, especially for those who use it for recreational activity. Private resort owners are highly encouraged to regularly monitor and maintain hot spring resorts to avoid future infections.
天然温泉是为人们和不同类型的微生物提供放松的理想场所和环境基质。从五个已确定的采集点共采集了 40 个地表水样本，每个点采集 8 个水样。采集点之间的距离相隔 200 米，以覆盖整个研究地点。地表水样本是从表面采集约 10-20 厘米。水样经过过滤、培养和 14 天的显微镜观察。培养 14 天后，有 8 个（20%）水样显示出囊和滋养体阶段。使用 JDP1 和 JDP2 特异性引物的聚合酶链反应证实了温泉中有两种来自我们的分离株的 spp.，分离株 1.1 和 5.1。进一步的测序表明，分离株是 T20 和 基因型 T7。序列被提交到 GenBank，并分别被分配到 PP741726 和 PP741727 的注册号。温泉中分离出的 spp.对健康有重大影响，特别是对那些将其用于娱乐活动的人。强烈鼓励私人度假胜地所有者定期监测和维护温泉胜地，以避免未来的感染。
